Fungal carbonyl sulfide hydrolase of trichoderma harzianum strain thif08 and its relationship with clade D β-carbonic anhydrases

Yoshihito Masaki, Ryuka Iizuka, Hiromi Kato, Yuka Kojima, Takahiro Ogawa, Makoto Yoshida, Yasuhiko Matsushita, Yoko Katayama

Research output: Contribution to journalArticlepeer-review

Abstract

Carbonyl sulfide (COS) is the most abundant and long-lived sulfur-containing gas in the atmosphere. Soil is the main sink of COS in the atmosphere and uptake is dominated by soil microorganisms; however, biochemical research has not yet been conducted on fungal COS degradation. COS hydrolase (COSase) was purified from Trichoderma harzianum strain THIF08, which degrades COS at concentrations higher than 10,000 parts per million by volume from atmospheric concentrations, and its gene cos (492 bp) was cloned. The recombinant protein purified from Escherichia coli expressing the cos gene converted COS to H2S. The deduced amino acid sequence of COSase (163 amino acids) was assigned to clade D in the phylogenetic tree of the β-carbonic anhydrase (β-CA) family, to which prokaryotic COSase and its structurally related enzymes belong. However, the COSase of strain THIF08 differed from the previously known prokaryotic COSase and its related enzymes due to its low reactivity to CO2 and inability to hydrolyze CS2. Sequence comparisons of the active site amino acids of clade D β-CA family enzymes suggested that various Ascomycota, particularly Sordariomycetes and Eurotiomycetes, possess similar enzymes to the COSase of strain THIF08 with >80% identity. These fungal COSase were phylogenetically distant to prokaryotic clade D β-CA family enzymes. These results suggest that various ascomycetes containing COSase contribute to the uptake of COS by soil.

Original languageEnglish
Article numberME20058
JournalMicrobes and environments
Volume36
Issue number2
DOIs
Publication statusPublished - 2021

Keywords

  • COS hydrolase
  • Carbonyl sulfide
  • Clade D β-carbonic anhydrase
  • Trichoderma harzianum

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Soil Science
  • Plant Science

Fingerprint

Dive into the research topics of 'Fungal carbonyl sulfide hydrolase of trichoderma harzianum strain thif08 and its relationship with clade D β-carbonic anhydrases'. Together they form a unique fingerprint.

Cite this