Functions of the 5'- and 3'-untranslated regions of tobamovirus RNA

Tetsuya Chujo, Kazuhiro Ishibashi, Shuhei Miyashita, Masayuki Ishikawa

Research output: Contribution to journalReview articlepeer-review

11 Citations (Scopus)

Abstract

The tobamovirus genome is a 5'-m7G-capped RNA that carries a tRNA-like structure at its 3'-terminus. The genomic RNA serves as the template for both translation and negative-strand RNA synthesis. The 5'- and 3'-untranslated regions (UTRs) of the genomic RNA contain elements that enhance translation, and the 3'-UTR also contains the elements necessary for the initiation of negative-strand RNA synthesis. Recent studies using a cell-free viral RNA translation-replication system revealed that a 70-nucleotide region containing a part of the 5'-UTR is bound cotranslationally by tobacco mosaic virus (TMV) replication proteins translated from the genomic RNA and that the binding leads the genomic RNA to RNA replication pathway. This mechanism explains the cis-preferential replication of TMV by the replication proteins. The binding also inhibits further translation to avoid a fatal ribosome-RNA polymerase collision, which might arise if translation and negative-strand synthesis occur simultaneously on a single genomic RNA molecule. Therefore, the 5'- and 3'-UTRs play multiple important roles in the life cycle of tobamovirus.

Original languageEnglish
Pages (from-to)82-89
Number of pages8
JournalVirus Research
Volume206
DOIs
Publication statusPublished - 2015 Aug 3
Externally publishedYes

Keywords

  • RNA
  • Replication
  • Tobacco mosaic virus
  • Tomato mosaic virus
  • Translation
  • Untranslated region

ASJC Scopus subject areas

  • Cancer Research
  • Virology
  • Infectious Diseases

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