Functionalized fullerene as an artificial vector for transfection

Eiichi Nakamura, Hiroyuki Isobe, Naoki Tomita, Masaya Sawamura, Shigeki Jinno, Hiroto Okayama

    Research output: Contribution to journalArticlepeer-review

    138 Citations (Scopus)

    Abstract

    Duplex DNA is bound by a 'two-handed' fullerene (see picture) which allows it to be expressed in mammalian cells. The molecular design relies on the synergy of the hydrophobic fullerene core and the two spatially separated cationic residues. Upon complexation with the fullerene the plasmid (containing a green fluorescent protein reporter gene) is taken up by cultured cells through phagocytosis and expressed in up to 15% of these cells.

    Original languageEnglish
    Pages (from-to)4254-4257
    Number of pages4
    JournalAngewandte Chemie - International Edition
    Volume39
    Issue number23
    DOIs
    Publication statusPublished - 2000 Dec 4

    Keywords

    • DNA recognition
    • Fullerenes
    • Polycations
    • Supramolecular chemistry
    • Transfection

    ASJC Scopus subject areas

    • Catalysis
    • Chemistry(all)

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