Functionalized fullerene as an artificial vector for transfection

Eiichi Nakamura; Hiroyuki Isobe; Naoki Tomita; Masaya Sawamura; Shigeki Jinno; Hiroto Okayama

doi:10.1002/1521-3773(20001201)39:23<4254::AID-ANIE4254>3.0.CO;2-O

Functionalized fullerene as an artificial vector for transfection

Eiichi Nakamura, Hiroyuki Isobe, Naoki Tomita, Masaya Sawamura, Shigeki Jinno, Hiroto Okayama

Research output: Contribution to journal › Article › peer-review

138 Citations (Scopus)

Abstract

Duplex DNA is bound by a 'two-handed' fullerene (see picture) which allows it to be expressed in mammalian cells. The molecular design relies on the synergy of the hydrophobic fullerene core and the two spatially separated cationic residues. Upon complexation with the fullerene the plasmid (containing a green fluorescent protein reporter gene) is taken up by cultured cells through phagocytosis and expressed in up to 15% of these cells.

Original language	English
Pages (from-to)	4254-4257
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	39
Issue number	23
DOIs	https://doi.org/10.1002/1521-3773(20001201)39:23<4254::AID-ANIE4254>3.0.CO;2-O
Publication status	Published - 2000 Dec 4

Keywords

DNA recognition
Fullerenes
Polycations
Supramolecular chemistry
Transfection

ASJC Scopus subject areas

Catalysis
Chemistry(all)

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10.1002/1521-3773(20001201)39:23<4254::AID-ANIE4254>3.0.CO;2-O

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AU - Nakamura, Eiichi

AU - Isobe, Hiroyuki

AU - Tomita, Naoki

AU - Sawamura, Masaya

AU - Jinno, Shigeki

AU - Okayama, Hiroto

PY - 2000/12/4

Y1 - 2000/12/4

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AB - Duplex DNA is bound by a 'two-handed' fullerene (see picture) which allows it to be expressed in mammalian cells. The molecular design relies on the synergy of the hydrophobic fullerene core and the two spatially separated cationic residues. Upon complexation with the fullerene the plasmid (containing a green fluorescent protein reporter gene) is taken up by cultured cells through phagocytosis and expressed in up to 15% of these cells.

KW - DNA recognition

KW - Fullerenes

KW - Polycations

KW - Supramolecular chemistry

KW - Transfection

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Functionalized fullerene as an artificial vector for transfection

Abstract

Keywords

ASJC Scopus subject areas

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