Functionalization of magnetic gold/iron-oxide composite nanoparticles with oligonucleotides and magnetic separation of specific target

Takuya Kinoshita; Satoshi Seino; Yoshiteru Mizukoshi; Takashi Nakagawa; Takao A. Yamamoto

doi:10.1016/j.jmmm.2006.10.1161

Functionalization of magnetic gold/iron-oxide composite nanoparticles with oligonucleotides and magnetic separation of specific target

Takuya Kinoshita, Satoshi Seino, Yoshiteru Mizukoshi, Takashi Nakagawa, Takao A. Yamamoto

Trans-Regional Corporation Center for Industrial Materials Research

Research output: Contribution to journal › Article › peer-review

40 Citations (Scopus)

Abstract

Magnetic composite nanoparticles of gold and iron-oxide synthesized with gamma-rays or ultrasonics were functionalized with thiol-modified oligonucleotides. The amount of oligonucleotides bound to the functionalized nanoparticle probes via hybridization was quantified with fluorescently-labeled target oligonucleotides. Our composite nanoparticles magnetically separated the specific target oligonucleotides without the non-specific adsorption.

Original language	English
Pages (from-to)	255-258
Number of pages	4
Journal	Journal of Magnetism and Magnetic Materials
Volume	311
Issue number	1 SPEC. ISS.
DOIs	https://doi.org/10.1016/j.jmmm.2006.10.1161
Publication status	Published - 2007 Apr

Keywords

Composite nanoparticle
Gold
Iron-oxide
Magnetic separation
Oligonucleotide

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics

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10.1016/j.jmmm.2006.10.1161

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Functionalization of magnetic gold/iron-oxide composite nanoparticles with oligonucleotides and magnetic separation of specific target. / Kinoshita, Takuya; Seino, Satoshi; Mizukoshi, Yoshiteru; Nakagawa, Takashi; Yamamoto, Takao A.

In: Journal of Magnetism and Magnetic Materials, Vol. 311, No. 1 SPEC. ISS., 04.2007, p. 255-258.

Research output: Contribution to journal › Article › peer-review

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title = "Functionalization of magnetic gold/iron-oxide composite nanoparticles with oligonucleotides and magnetic separation of specific target",

abstract = "Magnetic composite nanoparticles of gold and iron-oxide synthesized with gamma-rays or ultrasonics were functionalized with thiol-modified oligonucleotides. The amount of oligonucleotides bound to the functionalized nanoparticle probes via hybridization was quantified with fluorescently-labeled target oligonucleotides. Our composite nanoparticles magnetically separated the specific target oligonucleotides without the non-specific adsorption.",

keywords = "Composite nanoparticle, Gold, Iron-oxide, Magnetic separation, Oligonucleotide",

author = "Takuya Kinoshita and Satoshi Seino and Yoshiteru Mizukoshi and Takashi Nakagawa and Yamamoto, {Takao A.}",

note = "Funding Information: The authors thank Dr. N. Ohnishi (Magnabeat Inc.) and Prof. T. Doi (Osaka University) for their advice. This work was partially supported by Industrial Technology Research Program in 2003 from New Energy and Industrial Technology Development Organization (NEDO).",

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AU - Kinoshita, Takuya

AU - Seino, Satoshi

AU - Mizukoshi, Yoshiteru

AU - Nakagawa, Takashi

AU - Yamamoto, Takao A.

N1 - Funding Information: The authors thank Dr. N. Ohnishi (Magnabeat Inc.) and Prof. T. Doi (Osaka University) for their advice. This work was partially supported by Industrial Technology Research Program in 2003 from New Energy and Industrial Technology Development Organization (NEDO).

PY - 2007/4

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N2 - Magnetic composite nanoparticles of gold and iron-oxide synthesized with gamma-rays or ultrasonics were functionalized with thiol-modified oligonucleotides. The amount of oligonucleotides bound to the functionalized nanoparticle probes via hybridization was quantified with fluorescently-labeled target oligonucleotides. Our composite nanoparticles magnetically separated the specific target oligonucleotides without the non-specific adsorption.

AB - Magnetic composite nanoparticles of gold and iron-oxide synthesized with gamma-rays or ultrasonics were functionalized with thiol-modified oligonucleotides. The amount of oligonucleotides bound to the functionalized nanoparticle probes via hybridization was quantified with fluorescently-labeled target oligonucleotides. Our composite nanoparticles magnetically separated the specific target oligonucleotides without the non-specific adsorption.

KW - Composite nanoparticle

KW - Gold

KW - Iron-oxide

KW - Magnetic separation

KW - Oligonucleotide

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Functionalization of magnetic gold/iron-oxide composite nanoparticles with oligonucleotides and magnetic separation of specific target

Abstract

Keywords

ASJC Scopus subject areas

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