Functional TLRs and NODs in human gingival fibroblasts

A. Uehara, H. Takada

Research output: Contribution to journalArticlepeer-review

107 Citations (Scopus)

Abstract

Since human gingival fibroblasts are the major cells in periodontal tissues, we hypothesized that gingival fibroblasts are endowed with receptors for bacterial components, which induce innate immune responses against invading bacteria. We found clear mRNA expression of Toll-like receptors (TLR)1, TLR2, TLR3, TLR4, TLR5, TLR6, TLR7, TLR8, TLR9, MD-2, MyD88, NOD1, and NOD2 in gingival fibroblasts. Gingival fibroblasts constitutively expressed these molecules. Upon stimulation with chemically synthesized ligands mimicking microbial products for these receptors, the production of proinflammatory cytokines, such as interleukin (IL)-6, IL-8, and monocyte chemoattractant protein-1, was markedly up-regulated. Furthermore, the production of pro-inflammatory cytokines induced by TLR and NOD ligands was significantly inhibited by an RNA interference assay targeted to NF-κB. These findings indicate that these innate immunity-related molecules in gingival fibroblasts are functional receptors involved in inflammatory reactions in periodontal tissues, which might be responsible for periodontal pathogenesis.

Original languageEnglish
Pages (from-to)249-254
Number of pages6
JournalJournal of dental research
Volume86
Issue number3
DOIs
Publication statusPublished - 2007 Mar

Keywords

  • Fibroblasts
  • Innate immunity
  • NOD1
  • NOD2
  • TLR

ASJC Scopus subject areas

  • Dentistry(all)

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