TY - JOUR
T1 - Functional screening of p53 status in tumor cells using saccharomyces cervisiae
AU - Jia, L. Q.
AU - Ishioka, C.
AU - Osada, M.
AU - Gamo, M.
AU - Shimodaira, H.
AU - Suzuki, T.
AU - Kanamaru, R.
PY - 1997/9/24
Y1 - 1997/9/24
N2 - We have detected both germ-line and somatic p53 mutations in lymphocytes, cell lines and tumor tissues using a functional analysis of p53 tumor suppressor gene based on yeast transcription assay. Through our screening projects of the p53 gene, a number of missense p53 mutations were identified as loss-of-function mutations. This method, previously termed FASAY, is rapid, sensitive, less -expensive and can be automated for screening both somatic and germ-line p53 mutations.
AB - We have detected both germ-line and somatic p53 mutations in lymphocytes, cell lines and tumor tissues using a functional analysis of p53 tumor suppressor gene based on yeast transcription assay. Through our screening projects of the p53 gene, a number of missense p53 mutations were identified as loss-of-function mutations. This method, previously termed FASAY, is rapid, sensitive, less -expensive and can be automated for screening both somatic and germ-line p53 mutations.
KW - Yeast functional assay
KW - p53 tumor suppressor gene
UR - http://www.scopus.com/inward/record.url?scp=0030883483&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030883483&partnerID=8YFLogxK
M3 - Article
C2 - 9063485
AN - SCOPUS:0030883483
VL - 24
SP - 466
EP - 470
JO - Japanese Journal of Cancer and Chemotherapy
JF - Japanese Journal of Cancer and Chemotherapy
SN - 0385-0684
IS - 4
ER -
