Functional screening of p53 status in tumor cells using saccharomyces cervisiae

L. Q. Jia, C. Ishioka, M. Osada, M. Gamo, H. Shimodaira, T. Suzuki, R. Kanamaru

Research output: Contribution to journalArticlepeer-review

Abstract

We have detected both germ-line and somatic p53 mutations in lymphocytes, cell lines and tumor tissues using a functional analysis of p53 tumor suppressor gene based on yeast transcription assay. Through our screening projects of the p53 gene, a number of missense p53 mutations were identified as loss-of-function mutations. This method, previously termed FASAY, is rapid, sensitive, less -expensive and can be automated for screening both somatic and germ-line p53 mutations.

Original languageEnglish
Pages (from-to)466-470
Number of pages5
JournalJapanese Journal of Cancer and Chemotherapy
Volume24
Issue number4
Publication statusPublished - 1997 Sep 24

Keywords

  • Yeast functional assay
  • p53 tumor suppressor gene

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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