Functional replacement of Drosophila Btk29A with human Btk in male genital development and survival

Noriko Hamada; Carl Magnus Bäckesjö; C. I.Edvard Smith; Daisuke Yamamoto

doi:10.1016/j.febslet.2005.06.042

Functional replacement of Drosophila Btk29A with human Btk in male genital development and survival

Noriko Hamada, Carl Magnus Bäckesjö, C. I.Edvard Smith, Daisuke Yamamoto

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

Drosophila type 2 Btk29A reveals the highest homology to Btk among mammalian Tec kinases. In Btk29A^ficP mutant males, the apodeme holding the penis split into two pieces. Human Btk rescued this phenotype in 39% of Btk29A^ficP males, while the Drosophila transgenes did so in 90-100% of mutants. The Btk29A^ficP mutation reduced adult longevity to 11% that of wild-type. This effect was counteracted by Drosophila type 2, yielding 76% of the wild-type lifespan. Human Btk extended the lifespan of Btk29A^ficP mutants only to 20% that of wild-type. Thus human Btk can partially replace Drosophila Btk29A⁺ in male genital development and survival.

Original language	English
Pages (from-to)	4131-4137
Number of pages	7
Journal	FEBS Letters
Volume	579
Issue number	19
DOIs	https://doi.org/10.1016/j.febslet.2005.06.042
Publication status	Published - 2005 Aug 1

Keywords

Bruton's tyrosine kinase
Drosophila
Evolutionary conservation
Genital development
Longevity

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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title = "Functional replacement of Drosophila Btk29A with human Btk in male genital development and survival",

abstract = "Drosophila type 2 Btk29A reveals the highest homology to Btk among mammalian Tec kinases. In Btk29AficP mutant males, the apodeme holding the penis split into two pieces. Human Btk rescued this phenotype in 39% of Btk29AficP males, while the Drosophila transgenes did so in 90-100% of mutants. The Btk29AficP mutation reduced adult longevity to 11% that of wild-type. This effect was counteracted by Drosophila type 2, yielding 76% of the wild-type lifespan. Human Btk extended the lifespan of Btk29AficP mutants only to 20% that of wild-type. Thus human Btk can partially replace Drosophila Btk29A+ in male genital development and survival.",

keywords = "Bruton's tyrosine kinase, Drosophila, Evolutionary conservation, Genital development, Longevity",

author = "Noriko Hamada and B{\"a}ckesj{\"o}, {Carl Magnus} and Smith, {C. I.Edvard} and Daisuke Yamamoto",

note = "Funding Information: We thank Dr. M. Erd{\'e}lyi for the poirot stock of pcs and Ms. Aya Nishioka for secretarial assistance. This work was supported by Special Coordination Funds for Promoting Science and Technology from the Ministry of Education, Science, Sports and Culture of Japan to D.Y. and by the Swedish Cancer Fund, the Swedish Science Council and the Wallenberg Foundation to C.I.E.S.",

year = "2005",

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doi = "10.1016/j.febslet.2005.06.042",

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AU - Hamada, Noriko

AU - Bäckesjö, Carl Magnus

AU - Smith, C. I.Edvard

AU - Yamamoto, Daisuke

N1 - Funding Information: We thank Dr. M. Erdélyi for the poirot stock of pcs and Ms. Aya Nishioka for secretarial assistance. This work was supported by Special Coordination Funds for Promoting Science and Technology from the Ministry of Education, Science, Sports and Culture of Japan to D.Y. and by the Swedish Cancer Fund, the Swedish Science Council and the Wallenberg Foundation to C.I.E.S.

PY - 2005/8/1

Y1 - 2005/8/1

N2 - Drosophila type 2 Btk29A reveals the highest homology to Btk among mammalian Tec kinases. In Btk29AficP mutant males, the apodeme holding the penis split into two pieces. Human Btk rescued this phenotype in 39% of Btk29AficP males, while the Drosophila transgenes did so in 90-100% of mutants. The Btk29AficP mutation reduced adult longevity to 11% that of wild-type. This effect was counteracted by Drosophila type 2, yielding 76% of the wild-type lifespan. Human Btk extended the lifespan of Btk29AficP mutants only to 20% that of wild-type. Thus human Btk can partially replace Drosophila Btk29A+ in male genital development and survival.

AB - Drosophila type 2 Btk29A reveals the highest homology to Btk among mammalian Tec kinases. In Btk29AficP mutant males, the apodeme holding the penis split into two pieces. Human Btk rescued this phenotype in 39% of Btk29AficP males, while the Drosophila transgenes did so in 90-100% of mutants. The Btk29AficP mutation reduced adult longevity to 11% that of wild-type. This effect was counteracted by Drosophila type 2, yielding 76% of the wild-type lifespan. Human Btk extended the lifespan of Btk29AficP mutants only to 20% that of wild-type. Thus human Btk can partially replace Drosophila Btk29A+ in male genital development and survival.

KW - Bruton's tyrosine kinase

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KW - Evolutionary conservation

KW - Genital development

KW - Longevity

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