Functional quantitative analysis of the genome in cultured human mesangial cells

Yoshinari Yasuda, Toshio Miyata, Masaomi Nangaku, Yoshiyasu Iida, Kenji Maeda, Kiyoshi Kurokawa, Kousaku Okubo

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

For normal physiological function, each cell tightly regulates gene expression in a specific fashion so that critical proteins are synthesized in a well-coordinated manner. Therefore, it is very important to uncover which genes are expressed in specific cells. Recent technological advances combined with rapid large-scale DNA sequencing and computerized data processing have allowed us to investigate the expression levels of a variety of transcripts in the mesangial cells, a target of injury in many forms of glomerulonephritis. Utilizing a large scale sequencing of a 3'-directed cDNA library, which allows us to avoid variable cloning efficiencies reflecting the size of cDNA, we investigated expression profiles of various molecules in cultured human mesangial cells. Among the 1,193 sequenced clones, 688 (57.7%) appeared more than once (redundant sequence group), representing 203 different species. Thirty-nine of these appeared more than three times. The most abundant mRNA was that of fibronectin, which consisted of 3.9% of the total mRNA population. Except for mitochondrial or ribosomal genes, calcyclin came next (2.5%), followed by two cytoskeletal genes, γ-actin gene and calpactin 1 light chain gene, in addition to an amyloid precursor protein homolog (0.7%). In conclusion, we performed a molecular biological quantification of transcripts in mesangial cells. Fibronectin was the most abundantly expressed, followed by calcyclin, γ-actin, calpactin 1 light chain, and an amyloid precursor protein homolog. We also discovered some candidate genes specific for human mesangial cells. The expression profile of the transcripts serves as an important tool in understanding the biological properties of mesangial cells.

Original languageEnglish
Pages (from-to)154-158
Number of pages5
JournalKidney international
Volume53
Issue number1
DOIs
Publication statusPublished - 1998

Keywords

  • 3'-directed cDNA library
  • DNA sequencing
  • Expression profile
  • Genome analysis

ASJC Scopus subject areas

  • Nephrology

Fingerprint Dive into the research topics of 'Functional quantitative analysis of the genome in cultured human mesangial cells'. Together they form a unique fingerprint.

Cite this