Functional map of the α subunit of Escherichia coli RNA polymerase: Two modes of transcription activation by positive factors

K. Igarashi, A. Hanamura, K. Makino, H. Aiba, H. Aiba, T. Mizuno, A. Nakata, A. Ishihama

Research output: Contribution to journalArticle

100 Citations (Scopus)

Abstract

The role of the α subunit of Escherichia coli RNA polymerase in transcription activation by positive factors was investigated using two reconstituted mutant RNA polymerases (containing C-terminally truncated α subunits) and three positive factors [the cAMP receptor protein (CRP), OmpR, and PhoB]. The mutant RNA polymerases did not respond to transcription activation by activator proteins that bind upstream of the respective promoters. Transcription by these mutant enzymes was, however, activated in the cases where activators bind to target sites that overlap the promoter -35 region. Two different mechanisms are proposed for the positive control of transcription by activator proteins, one requiring the C-terminal domain of the α subunit, and the other not requiring it.

Original languageEnglish
Pages (from-to)8958-8962
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume88
Issue number20
DOIs
Publication statusPublished - 1991 Jan 1
Externally publishedYes

Keywords

  • deletion mutant
  • positive control
  • protein-protein contact
  • subunit assembly
  • transcription factor

ASJC Scopus subject areas

  • General

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