Functional Fv fragment of an antibody specific for CD28: Fv-mediated co-stimulation of T cells

Shin Ichi Takemura, Ryutaro Asano, Kouhei Tsumoto, Takashi Arai, Naoki Sakurai, Hideaki Kodama, Hiroshi Yoshida, Yu Katayose, Masanori Suzuki, Seiki Matsuno, Toshio Kudo, Izumi Kumagai

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7 Citations (Scopus)

Abstract

The most predominant co-stimulation pathway, which is critical for T cell activation and proliferation, is the CD28-B7 pathway. The anti-CD28 monoclonal antibody (mAb) also provides a co-stimulatory signal to T cells. In order to construct a functional Fv fragment (complex of VH and VL domains) of anti-CD28 antibody using a bacterial expression system, cDNA encoding the variable regions of immunoglobulin from 15E8 hybridoma cells was cloned and expressed in Escherichia coli. The Fv fragment was obtained as a soluble protein from the periplasmic fraction and showed a binding pattern similar to parental IgG. The Fv fragment induced proliferation of peripheral blood mononuclear cells in the presence of anti-CD3 or anti-CD2 mAb and enhanced anti-tumor activity of anti-MUC1xanti-CD3 bispecific antibody when tested with lymphokine-activated killer cells with T cell phenotype. Thus, the anti-CD28 Fv fragment will be promising not only for the study of co-stimulation, but also for cancer immunotherapy. Copyright (C) 2000 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)266-271
Number of pages6
JournalFEBS Letters
Volume476
Issue number3
DOIs
Publication statusPublished - 2000 Jul 7

Keywords

  • Bacterial expression
  • CD28-B7
  • Co-stimulation
  • Fv
  • Immunotherapy

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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    Takemura, S. I., Asano, R., Tsumoto, K., Arai, T., Sakurai, N., Kodama, H., Yoshida, H., Katayose, Y., Suzuki, M., Matsuno, S., Kudo, T., & Kumagai, I. (2000). Functional Fv fragment of an antibody specific for CD28: Fv-mediated co-stimulation of T cells. FEBS Letters, 476(3), 266-271. https://doi.org/10.1016/S0014-5793(00)01741-5