Functional emergence of a column-like architecture in layer 5 of mouse somatosensory cortex in vivo

Kyo Koizumi, Masatoshi Inoue, Srikanta Chowdhury, Haruhiko Bito, Akihiro Yamanaka, Toru Ishizuka, Hiromu Yawo

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

To investigate how the functional architecture is organized in layer 5 (L5) of the somatosensory cortex of a mouse in vivo, the input–output relationship was investigated using an all-optical approach. The neural activity in L5 was optically recorded using a Ca 2+ sensor, R-CaMP2, through a microprism inserted in the cortex under two-photon microscopy, while the L5 was regionally excited using optogenetics. The excitability was spread around the blue-light irradiated region, but the horizontal propagation was limited to within a certain distance (λ < 130 μm from the center of the illumination spot). When two regions were photostimulated with a short interval, the excitability of each cluster was reduced. Therefore, a column-like architecture had functionally emerged with reciprocal inhibition through a minimal number of synaptic relays. This could generate a synchronous output from a region of L5 with simultaneous enhancement of the signal-to-noise ratio by silencing of the neighboring regions.

Original languageEnglish
Pages (from-to)65-77
Number of pages13
JournalJournal of Physiological Sciences
Volume69
Issue number1
DOIs
Publication statusPublished - 2019 Jan 22

Keywords

  • Ca imaging
  • Column
  • Optogenetics
  • Reciprocal inhibition
  • Self-organization
  • Two-photon microscopy

ASJC Scopus subject areas

  • Physiology

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