Functional characterization of 23 allelic variants of thiopurine S-methyltransferase gene (TPMT*2 - *24)

Shuta Ujiie, Takamitsu Sasaki, Michinao Mizugaki, Masaaki Ishikawa, Masahiro Hiratsuka

Research output: Contribution to journalArticlepeer-review

65 Citations (Scopus)

Abstract

Objective Thiopurine S-methyltransferase (TPMT) is an enzyme responsible for the detoxification of the widely used thiopurine drugs. TPMT is genetically polymorphic and is associated with large interindividual variations in thiopurine drug toxicity and therapeutic efficacy. In this study, we performed an in-vitro analysis of TPMT variant alleles, namely, TPMT*2, *3A, *3B, *3C, *5, *6, *7, *8, *9, *10, *11, *12, *13, *14, *16, *17, *18, *19, *20, *21, *22, *23, and *24. Methods The wild-type TPMT proteins, TPMT.1 and 23 variants were heterologously expressed in COS-7 cells, and the kinetic parameters Km, V max, and intrinsic clearance (Vmax/Km) of 6-thioguanine S-methylation were determined. Results The expression levels of TPMT.2, TPMT.3A, TPMT.5, TPMT.12, TPMT.14, and TPMT.22 were considerably lower than that of TPMT.1 (P<0.005), and that of TPMT.18 was slightly reduced (P<0.05). The kinetic parameters of TPMT.3A, TPMT.3B, TPMT.5, TPMT.14, TPMT.18, TPMT.21, and TPMT.22 could not be accurately established because of no activity in 6-thioguanine S-methylation. The Vmax/Km values of TPMT.2, TPMT.7, TPMT.17, and TPMT.24 were displayed less than 10% of the wild-type. Conclusion This functional analysis with respect to TPMT variants could provide useful information for individualization of thiopurine drugs therapy.

Original languageEnglish
Pages (from-to)887-893
Number of pages7
JournalPharmacogenetics and Genomics
Volume18
Issue number10
DOIs
Publication statusPublished - 2008 Oct 1
Externally publishedYes

Keywords

  • Genetic polymorphism
  • Single nucleotide polymorphism
  • Thiopurine S-methyltransferase

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Genetics
  • Genetics(clinical)

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