Functional assay of EFA6A, a guanine nucleotide exchange factor for ADP-ribosylation factor 6 (ARF6), in dendritic formation of hippocampal neurons

Hiroyuki Sakagami, Akifumi Kamata, Kohji Fukunaga, Hisatake Kondo

Research output: Contribution to journalReview articlepeer-review

22 Citations (Scopus)

Abstract

EFA6A is a guanine nucleotide exchange factor (GEF) that can activate ADP-ribosylation factor 6 (ARF6) in vitro, with prominent expression in the forebrain including the hippocampal formation. In this section, we describe the neuronal transfection method and show that the overexpression of a catalytically inactive mutant of EFA6A induces a prominent dendritic formation of the primary hippocampal neurons, suggesting the intimate involvement of EFA6A in the regulation of neuronal dendritic development. This reliable and consistent neuronal transfection method will also be applicable for the vector-based RNA interference method.

Original languageEnglish
Article number22
Pages (from-to)232-242
Number of pages11
JournalMethods in enzymology
Volume404
DOIs
Publication statusPublished - 2005

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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