Functional annotation of intrinsically disordered domains by their amino acid content using IDD navigator

Ashwini Patil, Shunsuke Teraguchi, Huy Dinh, Kenta Nakai, Daron M. Standley

Research output: Contribution to journalConference article

4 Citations (Scopus)

Abstract

Function prediction of intrinsically disordered domains (IDDs) using sequence similarity methods is limited by their high mutability and prevalence of low complexity regions. We describe a novel method for identifying similar IDDs by a similarity metric based on amino acid composition and identify significantly overrepresented Gene Ontology (GO) and Pfam domain annotations within highly similar IDDs. Applications and extensions of the proposed method are discussed, in particular with respect to protein functional annotation. We test the predicted annotations in a large-scale survey of IDDs in mouse and find that the proposed method provides significantly greater protein coverage in terms of function prediction than traditional sequence alignment methods like BLAST. As a proof of concept we examined several disorder-containing proteins: GRA15 and ROP16, both encoded in the parasitic protozoa T. gondii; Cyclon, a mostly uncharacterized protein involved in the regulation of immune cell death; STIM1, a protein essential for regulating calcium levels in the endoplasmic reticulum. We show that the overrepresented GO terms are consistent with recently-reported biological functions. We implemented the method in the web server IDD Navigator. IDD Navigator is available at http://sysimm.ifrec.Osaka-u.ac.jp/disorder/beta.php.

Original languageEnglish
Pages (from-to)164-175
Number of pages12
JournalPacific Symposium on Biocomputing
Publication statusPublished - 2012 Jan 1
Event17th Pacific Symposium on Biocomputing, PSB 2012 - Kohala Coast, United States
Duration: 2012 Jan 32012 Jan 7

ASJC Scopus subject areas

  • Biomedical Engineering
  • Computational Theory and Mathematics

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