TY - JOUR
T1 - Functional and Genomic Characterization of Ligilactobacillus salivarius TUCO-L2 Isolated From Lama glama Milk
T2 - A Promising Immunobiotic Strain to Combat Infections
AU - Quilodrán-Vega, Sandra
AU - Albarracin, Leonardo
AU - Mansilla, Flavia
AU - Arce, Lorena
AU - Zhou, Binghui
AU - Islam, Md Aminul
AU - Tomokiyo, Mikado
AU - Al Kassaa, Imad
AU - Suda, Yoshihito
AU - Kitazawa, Haruki
AU - Villena, Julio
N1 - Funding Information:
This study was supported by ANPCyT–FONCyT grant PICT-2016-0410 to JV, grants from the project of NARO Bio-oriented Technology Research Advancement Institution (research program on the development of innovative technology, no. 01002A), and by a Grant-in-Aid for Scientific Research (A) (19H00965) from the Japan Society for the Promotion of Science (JSPS) to HK. This work was also supported by JSPS Core-to-Core Program, A. Advanced Research Networks entitled Establishment of international agricultural immunology research-core for a quantum improvement in food safety. MI was supported by JSPS (Postdoctoral Fellowship for Foreign Researchers, Program No. 18F18081). MT was supported by Tohoku University Global Hagi Scholarship.
Funding Information:
Funding. This study was supported by ANPCyT?FONCyT grant PICT-2016-0410 to JV, grants from the project of NARO Bio-oriented Technology Research Advancement Institution (research program on the development of innovative technology, no. 01002A), and by a Grant-in-Aid for Scientific Research (A) (19H00965) from the Japan Society for the Promotion of Science (JSPS) to HK. This work was also supported by JSPS Core-to-Core Program, A. Advanced Research Networks entitled Establishment of international agricultural immunology research-core for a quantum improvement in food safety. MI was supported by JSPS (Postdoctoral Fellowship for Foreign Researchers, Program No. 18F18081). MT was supported by Tohoku University Global Hagi Scholarship.
Publisher Copyright:
© Copyright © 2020 Quilodrán-Vega, Albarracin, Mansilla, Arce, Zhou, Islam, Tomokiyo, Al Kassaa, Suda, Kitazawa and Villena.
PY - 2020/12/8
Y1 - 2020/12/8
N2 - Potential probiotic or immunobiotic effects of lactic acid bacteria (LAB) isolated from the milk of the South American camelid llama (Lama glama) have not been reported in published studies. The aim of the present work was to isolate beneficial LAB from llama milk that can be used as potential probiotics active against bacterial pathogens. LAB strains were isolated from llama milk samples. In vitro functional characterization of the strains was performed by evaluating the resistance against gastrointestinal conditions and inhibition of the pathogen growth. Additionally, the adhesive and immunomodulatory properties of the strains were assessed. The functional studies were complemented with a comparative genomic evaluation and in vivo studies in mice. Ligilactobacillus salivarius TUCO-L2 showed enhanced probiotic/immunobiotic potential compared to that of other tested strains. The TUCO-L2 strain was resistant to pH and high bile salt concentrations and demonstrated antimicrobial activity against Gram-negative intestinal pathogens and adhesion to mucins and epithelial cells. L. salivarius TUCO-L2 modulated the innate immune response triggered by Toll-like receptor (TLR)-4 activation in intestinal epithelial cells. This effect involved differential regulation of the expression of inflammatory cytokines and chemokines mediated by the modulation of the negative regulators of the TLR signaling pathway. Moreover, the TUCO-L2 strain enhanced the resistance of mice to Salmonella infection. This is the first report on the isolation and characterization of a potential probiotic/immunobiotic strain from llama milk. The in vitro, in vivo, and in silico investigation performed in this study reveals several research directions that are needed to characterize the TUCO-L2 strain in detail to position this strain as a probiotic or immunobiotic that can be used against infections in humans or animals, including llama.
AB - Potential probiotic or immunobiotic effects of lactic acid bacteria (LAB) isolated from the milk of the South American camelid llama (Lama glama) have not been reported in published studies. The aim of the present work was to isolate beneficial LAB from llama milk that can be used as potential probiotics active against bacterial pathogens. LAB strains were isolated from llama milk samples. In vitro functional characterization of the strains was performed by evaluating the resistance against gastrointestinal conditions and inhibition of the pathogen growth. Additionally, the adhesive and immunomodulatory properties of the strains were assessed. The functional studies were complemented with a comparative genomic evaluation and in vivo studies in mice. Ligilactobacillus salivarius TUCO-L2 showed enhanced probiotic/immunobiotic potential compared to that of other tested strains. The TUCO-L2 strain was resistant to pH and high bile salt concentrations and demonstrated antimicrobial activity against Gram-negative intestinal pathogens and adhesion to mucins and epithelial cells. L. salivarius TUCO-L2 modulated the innate immune response triggered by Toll-like receptor (TLR)-4 activation in intestinal epithelial cells. This effect involved differential regulation of the expression of inflammatory cytokines and chemokines mediated by the modulation of the negative regulators of the TLR signaling pathway. Moreover, the TUCO-L2 strain enhanced the resistance of mice to Salmonella infection. This is the first report on the isolation and characterization of a potential probiotic/immunobiotic strain from llama milk. The in vitro, in vivo, and in silico investigation performed in this study reveals several research directions that are needed to characterize the TUCO-L2 strain in detail to position this strain as a probiotic or immunobiotic that can be used against infections in humans or animals, including llama.
KW - Lama glama milk
KW - Ligilactobacillus salivarius TUCO-L2
KW - bacterial infection
KW - immunomodulation
KW - intestinal epithelia cell
KW - probiotic
UR - http://www.scopus.com/inward/record.url?scp=85098084331&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85098084331&partnerID=8YFLogxK
U2 - 10.3389/fmicb.2020.608752
DO - 10.3389/fmicb.2020.608752
M3 - Article
AN - SCOPUS:85098084331
VL - 11
JO - Frontiers in Microbiology
JF - Frontiers in Microbiology
SN - 1664-302X
M1 - 608752
ER -