Functional analysis of the tyrosinase gene and brown-locus protein gene promoters

Shigeki Shibahara

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Tyrosinase is a rate-limiting enzyme of melanin biosynthesis and the brown (b)-locus protein is responsible for the formation of black melanin rather than brown. To identify the cis-acting element(s) required for pigment cell-specific gene transcription, we analyzed the promoter function of two pi- ment cell-specific genes encoding mouse tyrosinase and b-locus protein using a cell-free transcription system prepared from mouse melanoma cells. Functional and structural analysis of both gene promoters reveals that three elements are conserved in both genes at equivalent positions, suggesting that these elements may be responsible for pigment cell-specific transcription. We discuss possible mechanisms for pigment cell-specific expression of the tyrosinase and b-locus protein genes.

Original languageEnglish
Pages (from-to)S146-S149
JournalJournal of Investigative Dermatology
Volume100
Issue number2 SUPPL.
DOIs
Publication statusPublished - 1993 Feb

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

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