Functional analysis of the tissue-engineered stomach wall

Yoshio Hori, Tatsuo Nakamura, Dai Kimura, Kenji Kaino, Yoshimochi Kurokawa, Susumu Satomi, Yasuhiko Shimizu

Research output: Contribution to journalArticlepeer-review

35 Citations (Scopus)

Abstract

We have established a method for in situ tissue engineering of the stomach in a canine model using an acellular collagen scaffold graft. The current study was conducted to evaluate the functional aspects of the tissueengineered stomach wall. The anterior wall of the stomach in beagle dogs was replaced with a collagen sponge scaffold measuring 4 × 4 cm. At 16 weeks after implantation, the animals were sacrificed and the stomach specimens were evaluated immunohistochemically and physiologically. Regeneration of the proton pump and thin muscle layer, which are essential for mechanical and chemical digestion by the stomach, was observed in the tissueengineered gastric tissue. However, acetylcholine-induced contraction was not observed in the tissue-engineered stomach wall. Although there is still room for improvement, the tissue-engineered stomach wall had a highly organized structure, and it is anticipated that this approach could eventually become an alternative for stomach reconstruction after gastrectomy.

Original languageEnglish
Pages (from-to)868-872
Number of pages5
JournalArtificial Organs
Volume26
Issue number10
DOIs
Publication statusPublished - 2002

Keywords

  • Collagen
  • Gastrointestinal tract
  • Reconstruction
  • Regenerative medicine
  • Stomach
  • Tissue engineering

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering

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