Functional analysis of the cDNA encoding human tyrosinase precursor

Atsushi Takeda, Yasushi Tomita, Shoji Okinaga, Hachiro Tagami, Shigeki Shibahara

Research output: Contribution to journalArticlepeer-review

58 Citations (Scopus)

Abstract

The DNA segment harboring the promoter region and the exon 1 of the human tyrosinase gene has been cloned and characterized. Sequence analysis reveals the amino-terminal half of tyrosinase molecule including a signal peptide, of which six amino acid residues are not represented in the tyrosinase cDNA, pHT γ 1 [Shibahara et al. (1988) Tohoku J. Exp. Med. 156, 403-414]. We therefore constructed the expression plasmid containing the human tyrosinase precursor cDNA, and introduced it into mouse amelanotic melanoma cells. Both tyrosine hydroxylase and dopa oxidase activities were expressed only in the cells transfected with such a full-length cDNA, providing direct evidence that tyrosinase actually possesses a dual catalytic activity.

Original languageEnglish
Pages (from-to)984-990
Number of pages7
JournalBiochemical and biophysical research communications
Volume162
Issue number3
DOIs
Publication statusPublished - 1989 Aug 15

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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