Functional analysis of synaptotagmin gene regulatory regions in two distantly related ascidian species

Jun Matsumoto, You Katsuyama, Yukio Ohtsuka, Patrick Lemaire, Yasushi Okamura

    Research output: Contribution to journalArticle

    5 Citations (Scopus)

    Abstract

    We have studied the structure and function of a promoter region of the Halocynthia synaptotagmin (Hr-Syt) gene, which is abundantly expressed in neuronal cells. Our previous analysis suggested that the expression of Hr-Syt is regulated by at least one epidermal and two neuronal regulatory regions. In this study, the regulatory regions of Hr-Syt promoter were further characterized by using two species of ascidians, Halocynthia roretzi and Ciona intestinalis embryos. A putative GATA transcription factor binding site in the epidermal regulatory region has ectodermal enhancer activity in the Halocynthia embryo. Neuronal expression of Hr-Syt was regulated by multiple redundant enhancer regions. Among these enhancer regions, a 200-bp (-2900/-2700) region drove the reporter expression in neurons in both species of ascidian. Although the synaptotagmin promoter sequences did not show overall similarity between Hr-Syt and Ciona synaptotagmin (Ci-Syt), 5′-upsteream two short sequences of Ci-Syt have similarity to the -2766/-2732 region of the Hr-Syt promoter. The homeodomain binding sites in this region are required for the neuronal enhancer activity. These results suggest that GATA and homeodomain transcription factors regulate the expression of synaptotagmin.

    Original languageEnglish
    Pages (from-to)543-552
    Number of pages10
    JournalDevelopment Growth and Differentiation
    Volume50
    Issue number7
    DOIs
    Publication statusPublished - 2008 Sep

    Keywords

    • Ascidian
    • Enhancer
    • Neuron
    • Promoter
    • Synaptotagmin

    ASJC Scopus subject areas

    • Developmental Biology
    • Cell Biology

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