Functional analysis of Ia antigen-bearing keratinocytes: Mixed skin lymphocyte culture between Ia antigen-bearing pam 212 cells and allogeneic and syngeneic splenic T cells

Setsuya Aiba, Hachiro Tagami

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Keratinocytes express la antigens in various skin disorders, although the biological role of these Ia antigen-bearing (Ia+) keratinocytes remains unclear. We induced Ia antigens on Pam 212 murine keratinocyte cellline by interferon-γ (IFN-γ) and using these cells, we performed the mixed skin lymphocyte culture with syngeneic BALB/c or allogeneic C3H/He splenic T cells. Unexpectedly, Pam 212 cells were found to stimulate both syngeneic and allogeneic T cells irrespective of IFN-γ treatment. However, both syngeneic and allogeneic T cells cultured with IFN-γ-treated Pam 212 cells incorporated [3H]thymidine much more actively than those cultured with IFN-γ-untreated Pam 212 cells. This stimulation was not inhibited by monoclonal anti-I-Ad antibody. Analysis of the responding T cells demonstrated that the syngeneic T-cell stimulation by IFN-γ-treated Pam 212 cells occurred in both purified Lyt 1- T cells and Lyt 2- T cells. Furthermore, we found that the T cells cultured with the IFN-γ-treated cells were composed of two morphologically different types of cells. Determination of their surface phenotype showed that the small cell population consisted of 57% Thy-1+, 23% Lyt-1+, 6% Lyt-2+, and 9% asialo-GM1+ cells, while the large cells consisted of 53% Thy-1+, 15% Lyt-1+, 9% Lyt-2+, and 24% asialoGM1+ cells. These findings suggest that IFN-γ-treated Pam 212 cells could stimulate more than one kind of splenic T cell populations.

Original languageEnglish
Pages (from-to)560-566
Number of pages7
JournalJournal of Investigative Dermatology
Volume89
Issue number6
DOIs
Publication statusPublished - 1987 Dec

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

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