Functional analysis of C2H2 zinc finger transcription factor CrzA involved in calcium signaling in Aspergillus nidulans

Daisuke Hagiwara, Atsushi Kondo, Tomonori Fujioka, Keietsu Abe

    Research output: Contribution to journalArticlepeer-review

    56 Citations (Scopus)

    Abstract

    Calcium signaling systems are widely employed in eukaryotes and are implicated in the regulation of diverse biological processes. Calcineurin is an important signaling component, which mediates ion homeostasis and virulence in several fungi. Based on intensive studies conducted on budding yeast, transcription factor Crz1p is thought to be a target of calcineurin. To provide insight into calcium signaling, a Crz1p homolog (CrzA) in a filamentous fungus Aspergillus nidulans was identified and its function with special reference to calcium response was characterized. A crzA gene disruption mutant exhibited sensitivity to high concentrations of Mn2+ and Ca2+, and mediated the expression of P-type calcium-ATPase homologous genes. Comprehensive transcriptional analysis with DNA microarrays indicated that CrzA regulates the expression of a vacuolar Ca2+/ H+ exchanger gene in response to external calcium stimuli. It is suggested that the calcineurin-CrzA pathway is the mediator of Ca2+ homeostasis in A. nidulans. Moreover, a crzA/hogA double mutant showed hypersensitivity to osmotic stress, indicating the importance of calcium homeostasis for adaptation to osmotic stress, a universal stress in filamentous fungi.

    Original languageEnglish
    Pages (from-to)325-338
    Number of pages14
    JournalCurrent Genetics
    Volume54
    Issue number6
    DOIs
    Publication statusPublished - 2008

    Keywords

    • Aspergillus nidulans
    • Calcineurin
    • Calcium signaling
    • DNA microarray
    • Hog AMAPK
    • P-type ATPase
    • crzA

    ASJC Scopus subject areas

    • Genetics

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