Functional analysis of cDNAs for two types of human heme oxygenase and evidence for their separate regulation

Shigeki Shibahara, Miki Yoshizawa, Hiroyuki Suzuki, Kazuhisa Takeda, Kuniaki Meguro, Kazuyasu Endo

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171 Citations (Scopus)

Abstract

We cloned a cDNA coding for a putative human heme oxygenase isozyme, designated type 2 (HO-2), and analyzed its function by transient expression assays. HeLa cells transfected with either HO-2 cDNAs or a cDNA coding for authentic heme oxygenase (HO-1) expressed the activity of heme oxygenase, although no activity was detected in the mock transfected cells. Using specific anti-HO-1 antibody, we showed that expression of a HO-1 cDNA resulted in the increase in its protein levels, but HO-1 protein was not detectable in the cells expressing HO-2 cDNAs. We thus confirmed the functional identity of HO-1 and HO-2. Then, we analyzed their expression in an erythroid cell line, YN-1-0-A. Treatment with hem in or by heat shock (42°C) led to a remarkable increase in the HO-1 mRNA levels, while HO-2 mRNA expression was not induced at all, suggesting that they are under separate regulation. 1993

Original languageEnglish
Pages (from-to)214-218
Number of pages5
JournalJournal of biochemistry
Volume113
Issue number2
DOIs
Publication statusPublished - 1993 Feb

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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