Functional analysis of AtHKT1 in Arabidopsis shows that Na+ recirculation by the phloem is crucial for salt tolerance

Pierre Berthomieu, Geneviève Conéjéro, Aurélie Nublat, William J. Brackenbury, Cécile Lambert, Cristina Savio, Nobuyuki Uozumi, Shigetoshi Oiki, Katsuyuki Yamada, Françoise Cellier, Françoise Gosti, Thierry Simonneau, Pauline A. Essah, Mark Tester, Anne Aliénor Véry, Hervé Sentenac, Francine Casse

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Abstract

Two allelic recessive mutations of Arabidopsis, sas2-1 and sas2-2, were identified as inducing sodium overaccumulation in shoots. The sas2 locus was found (by positional cloning) to correspond to the AtHKT1 gene. Expression in Xenopus oocytes revealed that the sas2-1 mutation did not affect the ionic selectivity of the transporter but strongly reduced the macroscopic (whole oocyte current) transport activity. In Arabidopsis, expression of AtHKT1 was shown to be restricted to the phloem tissues in all organs. The sas2-1 mutation strongly decreased Na+ concentration in the phloem sap. It led to Na+ overaccumulation in every aerial organ (except the stem), but to Na+ underaccumulation in roots. The sas2 plants displayed increased sensitivity to NaCl, with reduced growth and even death under moderate salinity. The whole set of data indicates that AtHKT1 is involved in Na+ recirculation from shoots to roots, probably by mediating Na+ loading into the phloem sap in shoots and unloading in roots, this recirculation removing large amounts of Na+ from the shoot and playing a crucial role in plant tolerance to salt.

Original languageEnglish
Pages (from-to)2004-2014
Number of pages11
JournalEMBO Journal
Volume22
Issue number9
DOIs
Publication statusPublished - 2003 May 1

Keywords

  • Na exclusion
  • Na transport
  • Phloem
  • Positional cloning/salt tolerance

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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    Berthomieu, P., Conéjéro, G., Nublat, A., Brackenbury, W. J., Lambert, C., Savio, C., Uozumi, N., Oiki, S., Yamada, K., Cellier, F., Gosti, F., Simonneau, T., Essah, P. A., Tester, M., Véry, A. A., Sentenac, H., & Casse, F. (2003). Functional analysis of AtHKT1 in Arabidopsis shows that Na+ recirculation by the phloem is crucial for salt tolerance. EMBO Journal, 22(9), 2004-2014. https://doi.org/10.1093/emboj/cdg207