Functional analysis of an endo-1,6-β-D-glucanase gene (neg-1) from Neurospora crassa

Seiji Oyama, Hirokazu Inoue, Youhei Yamagata, Tasuku Nakajima, Keietu Abe

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

The 1,6-β-D-glucanase gene (neg1) of Neurospora crassa was disrupted by repeat-induced point mutations. Sequence analysis of the neg1 gene in the R12-1 mutant showed that 9 nucleotides within the coding region of the gene changed from GC to AT. The base transition of C to A at position 662 resulted in a codon. No apparent phenotypic changes were observed in the mutant, but Congo-red, SDS, and cetyltrimethyl ammonium bromide (CTAB), which affect fungal cell walls or membranes, markedly inhibited the hyphal growth of the mutant at a concentration that does not inhibit growth in the wild type.

Original languageEnglish
Pages (from-to)1773-1775
Number of pages3
JournalBioscience, Biotechnology and Biochemistry
Volume70
Issue number7
DOIs
Publication statusPublished - 2006 Aug 1

Keywords

  • 1,6-β-D-glucanase
  • Fungal cell wall
  • Fungal β-1,6-glucanase
  • β-1,6-glucanase disruptant
  • β-1,6-glucanase gene

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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