Functional Alteration of Macrophages by a Slime-Forming Lactococcus lactis ssp. cremoris

Haruki Kitazawa, Masahito Nomura, Takatoshi Itoh, Takahiro Yamaguchi

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

The effect of a slime-forming, encapsulated Lactococcus lactis ssp. cremoris KVS20 on macrophage function has been examined in vivo and in vitro in short-term studies. Peritoneal macrophages in which 21 to 34% of macrophage was presenting Fcγ-receptor positive macrophages were elicited by intraperitoneal injection of 10 to 50 mg/kg of L. lactis ssp. cremoris KVS20. The peritoneal macrophage exhibited cytotoxic activity against Sarcoma-180 cells in which the maximum activity was obtained in macrophage from mice injected with 10 mg/kg on d 5. However, L. lactis ssp. cremoris KVS20 rendered the elicited macrophage cytotoxic in vitro. The cytotoxicity was significantly augmented by 6- and 24-h treatment at the concentration of 50 to 500 μg/ml. These results obtained in the short-term studies demonstrated that the antitumor activity of L. lactis ssp. cremoris KVS20 may be mediated through the enhanced cytotoxic activity of macrophage.

Original languageEnglish
Pages (from-to)2082-2088
Number of pages7
JournalJournal of Dairy Science
Volume74
Issue number7
DOIs
Publication statusPublished - 1991

Keywords

  • EA
  • FCS
  • Fcγ-receptor positive macrophage(s)
  • FcγR-Mφ)
  • GVB
  • Lactococcus lactis ssp. cremoris KVS20
  • PE
  • PE-Mφ
  • PMN
  • S-180
  • SRBC
  • TG-Mφ
  • anti-SRBC IgG-coated SRBC
  • cytotoxicity
  • fetal calf serum
  • gelatin veronal buffer containing Ca and Mg
  • i.p.
  • intraperitoneal(ly)
  • macrophage function
  • macrophage(s)
  • peritoneal exudate
  • peritoneal macrophage(s)
  • polymorphonuclear cells
  • sarcoma-180
  • sheep red blood cells
  • thioglycolate-elicited macrophage(s)

ASJC Scopus subject areas

  • Food Science
  • Animal Science and Zoology
  • Genetics

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