Functional Alteration of Macrophages by a Slime-Forming Lactococcus lactis ssp. cremoris

Haruki Kitazawa, Masahito Nomura, Takatoshi Itoh, Takahiro Yamaguchi

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    23 Citations (Scopus)

    Abstract

    The effect of a slime-forming, encapsulated Lactococcus lactis ssp. cremoris KVS20 on macrophage function has been examined in vivo and in vitro in short-term studies. Peritoneal macrophages in which 21 to 34% of macrophage was presenting Fcγ-receptor positive macrophages were elicited by intraperitoneal injection of 10 to 50 mg/kg of L. lactis ssp. cremoris KVS20. The peritoneal macrophage exhibited cytotoxic activity against Sarcoma-180 cells in which the maximum activity was obtained in macrophage from mice injected with 10 mg/kg on d 5. However, L. lactis ssp. cremoris KVS20 rendered the elicited macrophage cytotoxic in vitro. The cytotoxicity was significantly augmented by 6- and 24-h treatment at the concentration of 50 to 500 μg/ml. These results obtained in the short-term studies demonstrated that the antitumor activity of L. lactis ssp. cremoris KVS20 may be mediated through the enhanced cytotoxic activity of macrophage.

    Original languageEnglish
    Pages (from-to)2082-2088
    Number of pages7
    JournalJournal of Dairy Science
    Volume74
    Issue number7
    DOIs
    Publication statusPublished - 1991

    Keywords

    • EA
    • FCS
    • Fcγ-receptor positive macrophage(s)
    • FcγR-Mφ)
    • GVB
    • Lactococcus lactis ssp. cremoris KVS20
    • PE
    • PE-Mφ
    • PMN
    • S-180
    • SRBC
    • TG-Mφ
    • anti-SRBC IgG-coated SRBC
    • cytotoxicity
    • fetal calf serum
    • gelatin veronal buffer containing Ca and Mg
    • i.p.
    • intraperitoneal(ly)
    • macrophage function
    • macrophage(s)
    • peritoneal exudate
    • peritoneal macrophage(s)
    • polymorphonuclear cells
    • sarcoma-180
    • sheep red blood cells
    • thioglycolate-elicited macrophage(s)

    ASJC Scopus subject areas

    • Food Science
    • Animal Science and Zoology
    • Genetics

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