Abstract
The effect of a slime-forming, encapsulated Lactococcus lactis ssp. cremoris KVS20 on macrophage function has been examined in vivo and in vitro in short-term studies. Peritoneal macrophages in which 21 to 34% of macrophage was presenting Fcγ-receptor positive macrophages were elicited by intraperitoneal injection of 10 to 50 mg/kg of L. lactis ssp. cremoris KVS20. The peritoneal macrophage exhibited cytotoxic activity against Sarcoma-180 cells in which the maximum activity was obtained in macrophage from mice injected with 10 mg/kg on d 5. However, L. lactis ssp. cremoris KVS20 rendered the elicited macrophage cytotoxic in vitro. The cytotoxicity was significantly augmented by 6- and 24-h treatment at the concentration of 50 to 500 μg/ml. These results obtained in the short-term studies demonstrated that the antitumor activity of L. lactis ssp. cremoris KVS20 may be mediated through the enhanced cytotoxic activity of macrophage.
Original language | English |
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Pages (from-to) | 2082-2088 |
Number of pages | 7 |
Journal | Journal of Dairy Science |
Volume | 74 |
Issue number | 7 |
DOIs | |
Publication status | Published - 1991 |
Keywords
- EA
- FCS
- Fcγ-receptor positive macrophage(s)
- FcγR-Mφ)
- GVB
- Lactococcus lactis ssp. cremoris KVS20
- Mφ
- PE
- PE-Mφ
- PMN
- S-180
- SRBC
- TG-Mφ
- anti-SRBC IgG-coated SRBC
- cytotoxicity
- fetal calf serum
- gelatin veronal buffer containing Ca and Mg
- i.p.
- intraperitoneal(ly)
- macrophage function
- macrophage(s)
- peritoneal exudate
- peritoneal macrophage(s)
- polymorphonuclear cells
- sarcoma-180
- sheep red blood cells
- thioglycolate-elicited macrophage(s)
ASJC Scopus subject areas
- Food Science
- Animal Science and Zoology
- Genetics