Function of type II phospholipase A2 in dopamine secretion by rat neuronal PC12 cells

Ichiro Kudo; Atsushi Matsuzawa; Kazuhiro Imai; Makoto Murakami; Keizo Inoue

doi:10.1016/0929-7855(96)01504-0

Function of type II phospholipase A₂ in dopamine secretion by rat neuronal PC12 cells

Ichiro Kudo, Atsushi Matsuzawa, Kazuhiro Imai, Makoto Murakami, Keizo Inoue

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

When rat pheochromocytoma PC12 cells, which had differentiated into neuron-like cells following culture with nerve growth factor, were activated with glutamate, approximately 40% of their type II phospholipase A₂ (PLA₂) was released into the extracellular medium. Glutamate-stimulated secretion of dopamine from PC12 cells was suppressed by type II PLA₂ inhibitors. Exogenous type II PLA₂ added alone directly elicited release of dopamine from PC12 cells. Thus released type II PLA₂ may be involved in the regulation of neurotransmitter exocytosis by PC12 cells.

Original language	English
Pages (from-to)	25-31
Number of pages	7
Journal	Journal of Lipid Mediators and Cell Signalling
Volume	14
Issue number	1-3
DOIs	https://doi.org/10.1016/0929-7855(96)01504-0
Publication status	Published - 1996 Sep
Externally published	Yes

Keywords

PC12 cells
dopamine
exocytosis
glutamate
phospholipase A

ASJC Scopus subject areas

Immunology
Pharmacology

Access to Document

10.1016/0929-7855(96)01504-0

Cite this

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title = "Function of type II phospholipase A2 in dopamine secretion by rat neuronal PC12 cells",

abstract = "When rat pheochromocytoma PC12 cells, which had differentiated into neuron-like cells following culture with nerve growth factor, were activated with glutamate, approximately 40% of their type II phospholipase A2 (PLA2) was released into the extracellular medium. Glutamate-stimulated secretion of dopamine from PC12 cells was suppressed by type II PLA2 inhibitors. Exogenous type II PLA2 added alone directly elicited release of dopamine from PC12 cells. Thus released type II PLA2 may be involved in the regulation of neurotransmitter exocytosis by PC12 cells.",

keywords = "PC12 cells, dopamine, exocytosis, glutamate, phospholipase A",

author = "Ichiro Kudo and Atsushi Matsuzawa and Kazuhiro Imai and Makoto Murakami and Keizo Inoue",

note = "Funding Information: This work was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan.",

year = "1996",

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T1 - Function of type II phospholipase A2 in dopamine secretion by rat neuronal PC12 cells

AU - Kudo, Ichiro

AU - Matsuzawa, Atsushi

AU - Imai, Kazuhiro

AU - Murakami, Makoto

AU - Inoue, Keizo

N1 - Funding Information: This work was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan.

PY - 1996/9

Y1 - 1996/9

N2 - When rat pheochromocytoma PC12 cells, which had differentiated into neuron-like cells following culture with nerve growth factor, were activated with glutamate, approximately 40% of their type II phospholipase A2 (PLA2) was released into the extracellular medium. Glutamate-stimulated secretion of dopamine from PC12 cells was suppressed by type II PLA2 inhibitors. Exogenous type II PLA2 added alone directly elicited release of dopamine from PC12 cells. Thus released type II PLA2 may be involved in the regulation of neurotransmitter exocytosis by PC12 cells.

AB - When rat pheochromocytoma PC12 cells, which had differentiated into neuron-like cells following culture with nerve growth factor, were activated with glutamate, approximately 40% of their type II phospholipase A2 (PLA2) was released into the extracellular medium. Glutamate-stimulated secretion of dopamine from PC12 cells was suppressed by type II PLA2 inhibitors. Exogenous type II PLA2 added alone directly elicited release of dopamine from PC12 cells. Thus released type II PLA2 may be involved in the regulation of neurotransmitter exocytosis by PC12 cells.

KW - PC12 cells

KW - dopamine

KW - exocytosis

KW - glutamate

KW - phospholipase A

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