Function of in situ synthesized membrane protein in the presence of cell-size liposome

Wakiko Asayama; Satoshi Kondoh; Shin Ichiro M. Nomura; Kazunari Akiyoshi

Function of in situ synthesized membrane protein in the presence of cell-size liposome

Wakiko Asayama, Satoshi Kondoh, Shin Ichiro M. Nomura, Kazunari Akiyoshi

Research output: Contribution to conference › Paper › peer-review

Abstract

Membrane proteins perform significant functions such as metabolisms or signal transductions. However, because of their hydrophobicity, it is difficult and time-consuming to isolate and purify them with keeping function. A convenient method enabling to construct pure membrane protein is awaited. We investigated in vitro membrane protein synthesis in the presence of liposomes. Connexin is a component of gap-junctions that mediate intercellular communication. Cell-free membrane protein synthesis for connexin43(C×43) was carried out and functional protein(C×43) containing liposomes was directly obtained. The bidirectional transfer of water-soluble dye, calcein through gap junctions was observed between C×43-expressing liposomes and cultured cells. The efficiency was estimated about 30 %. These results also show the C×43-liposome's possibility for novel cytosolic delivery system.

Original language	English
Number of pages	1
Publication status	Published - 2006 Oct 19
Externally published	Yes
Event	55th SPSJ Annual Meeting - Nagoya, Japan Duration: 2006 May 24 → 2006 May 26

Other

Other	55th SPSJ Annual Meeting
Country/Territory	Japan
City	Nagoya
Period	06/5/24 → 06/5/26

Keywords

Drug delivery system (DDS)
Gap-junction
Liposome
Membrane protein

ASJC Scopus subject areas

Engineering(all)

Cite this

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title = "Function of in situ synthesized membrane protein in the presence of cell-size liposome",

abstract = "Membrane proteins perform significant functions such as metabolisms or signal transductions. However, because of their hydrophobicity, it is difficult and time-consuming to isolate and purify them with keeping function. A convenient method enabling to construct pure membrane protein is awaited. We investigated in vitro membrane protein synthesis in the presence of liposomes. Connexin is a component of gap-junctions that mediate intercellular communication. Cell-free membrane protein synthesis for connexin43(C×43) was carried out and functional protein(C×43) containing liposomes was directly obtained. The bidirectional transfer of water-soluble dye, calcein through gap junctions was observed between C×43-expressing liposomes and cultured cells. The efficiency was estimated about 30 %. These results also show the C×43-liposome's possibility for novel cytosolic delivery system.",

keywords = "Drug delivery system (DDS), Gap-junction, Liposome, Membrane protein",

author = "Wakiko Asayama and Satoshi Kondoh and Nomura, {Shin Ichiro M.} and Kazunari Akiyoshi",

year = "2006",

month = oct,

day = "19",

language = "English",

note = "55th SPSJ Annual Meeting ; Conference date: 24-05-2006 Through 26-05-2006",

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TY - CONF

T1 - Function of in situ synthesized membrane protein in the presence of cell-size liposome

AU - Asayama, Wakiko

AU - Kondoh, Satoshi

AU - Nomura, Shin Ichiro M.

AU - Akiyoshi, Kazunari

PY - 2006/10/19

Y1 - 2006/10/19

N2 - Membrane proteins perform significant functions such as metabolisms or signal transductions. However, because of their hydrophobicity, it is difficult and time-consuming to isolate and purify them with keeping function. A convenient method enabling to construct pure membrane protein is awaited. We investigated in vitro membrane protein synthesis in the presence of liposomes. Connexin is a component of gap-junctions that mediate intercellular communication. Cell-free membrane protein synthesis for connexin43(C×43) was carried out and functional protein(C×43) containing liposomes was directly obtained. The bidirectional transfer of water-soluble dye, calcein through gap junctions was observed between C×43-expressing liposomes and cultured cells. The efficiency was estimated about 30 %. These results also show the C×43-liposome's possibility for novel cytosolic delivery system.

AB - Membrane proteins perform significant functions such as metabolisms or signal transductions. However, because of their hydrophobicity, it is difficult and time-consuming to isolate and purify them with keeping function. A convenient method enabling to construct pure membrane protein is awaited. We investigated in vitro membrane protein synthesis in the presence of liposomes. Connexin is a component of gap-junctions that mediate intercellular communication. Cell-free membrane protein synthesis for connexin43(C×43) was carried out and functional protein(C×43) containing liposomes was directly obtained. The bidirectional transfer of water-soluble dye, calcein through gap junctions was observed between C×43-expressing liposomes and cultured cells. The efficiency was estimated about 30 %. These results also show the C×43-liposome's possibility for novel cytosolic delivery system.

KW - Drug delivery system (DDS)

KW - Gap-junction

KW - Liposome

KW - Membrane protein

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M3 - Paper

AN - SCOPUS:33749838939

T2 - 55th SPSJ Annual Meeting

Y2 - 24 May 2006 through 26 May 2006

ER -

Function of in situ synthesized membrane protein in the presence of cell-size liposome

Abstract

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Keywords

ASJC Scopus subject areas

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