Function of in situ synthesized membrane protein in the presence of cell-size liposome

Wakiko Asayama, Satoshi Kondoh, Shin Ichiro M. Nomura, Kazunari Akiyoshi

Research output: Contribution to conferencePaperpeer-review

Abstract

Membrane proteins perform significant functions such as metabolisms or signal transductions. However, because of their hydrophobicity, it is difficult and time-consuming to isolate and purify them with keeping function. A convenient method enabling to construct pure membrane protein is awaited. We investigated in vitro membrane protein synthesis in the presence of liposomes. Connexin is a component of gap-junctions that mediate intercellular communication. Cell-free membrane protein synthesis for connexin43(C×43) was carried out and functional protein(C×43) containing liposomes was directly obtained. The bidirectional transfer of water-soluble dye, calcein through gap junctions was observed between C×43-expressing liposomes and cultured cells. The efficiency was estimated about 30 %. These results also show the C×43-liposome's possibility for novel cytosolic delivery system.

Original languageEnglish
Number of pages1
Publication statusPublished - 2006 Oct 19
Externally publishedYes
Event55th SPSJ Annual Meeting - Nagoya, Japan
Duration: 2006 May 242006 May 26

Other

Other55th SPSJ Annual Meeting
CountryJapan
CityNagoya
Period06/5/2406/5/26

Keywords

  • Drug delivery system (DDS)
  • Gap-junction
  • Liposome
  • Membrane protein

ASJC Scopus subject areas

  • Engineering(all)

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