TY - JOUR
T1 - Function of angiotensin II type 2 receptor in the postglomerular efferent arteriole
AU - Endo, Y.
AU - Arima, S.
AU - Yaoita, H.
AU - Omata, K.
AU - Tsunoda, K.
AU - Takeuchi, Kazuhisa
AU - Abe, K.
AU - Ito, S.
N1 - Copyright:
Copyright 2004 Elsevier Science B.V., Amsterdam. All rights reserved.
PY - 1997
Y1 - 1997
N2 - The balance of vasucular tone between afferent (Af-Art) and efferent arterioles (Ef-Art) is a critical determinant of the glomerular hemodynamics. We recently reported that selective activation of angiotensin II type 2 receptor (AT-2R) causes dilation in the Af-Art. However, the functional role of AT-2R in the Ef-Art has not been defined. In the present study, we microperfused rabbit Ef-Art in vitro, and examined the effect of angiotensin II (Ang II; 10-11 to 10-8 M) on the luminal diameter in the presence or absence of an AT-2R antagonist PD123319 (PD; 10-7 M). Angiotensin II caused dose-dependent constriction in Ef-Arts, with a significant constriction occurring at 10-11 M; at 10-10 M the diameter decreased by 28 ± 4% (N = 6). Pretreatment with PD significantly (P < 0.0125) augmented vasoconstrictor action of Ang II; at 10-10 M the diameter decreased by 44 ± 4% (N = 6). We then examined whether blockade of Ang II type 1 receptor (AT-1R) uncovers vasodilator action of Ang II mediated by AT-2R. Efferent arterioles were preconstricted by about 40% with norepinephrine and AT-1R was blocked with its selective antagonist CV11974 (CV; 10-8 M). Angiotensin II added to preconstricted and CV-pretreated Ef-Arts caused a dose-dependent dilation; at 10-8 M diameter increased by 28 ± 3% (N = 5). The dilation was completely abolished by simultaneous pretreatment with PD (N = 4). Our results demonstrate that in the Ef-Art selective activation of AT-2R causes vasodilation, which opposes the vasoconstrictor action of Ang II.
AB - The balance of vasucular tone between afferent (Af-Art) and efferent arterioles (Ef-Art) is a critical determinant of the glomerular hemodynamics. We recently reported that selective activation of angiotensin II type 2 receptor (AT-2R) causes dilation in the Af-Art. However, the functional role of AT-2R in the Ef-Art has not been defined. In the present study, we microperfused rabbit Ef-Art in vitro, and examined the effect of angiotensin II (Ang II; 10-11 to 10-8 M) on the luminal diameter in the presence or absence of an AT-2R antagonist PD123319 (PD; 10-7 M). Angiotensin II caused dose-dependent constriction in Ef-Arts, with a significant constriction occurring at 10-11 M; at 10-10 M the diameter decreased by 28 ± 4% (N = 6). Pretreatment with PD significantly (P < 0.0125) augmented vasoconstrictor action of Ang II; at 10-10 M the diameter decreased by 44 ± 4% (N = 6). We then examined whether blockade of Ang II type 1 receptor (AT-1R) uncovers vasodilator action of Ang II mediated by AT-2R. Efferent arterioles were preconstricted by about 40% with norepinephrine and AT-1R was blocked with its selective antagonist CV11974 (CV; 10-8 M). Angiotensin II added to preconstricted and CV-pretreated Ef-Arts caused a dose-dependent dilation; at 10-8 M diameter increased by 28 ± 3% (N = 5). The dilation was completely abolished by simultaneous pretreatment with PD (N = 4). Our results demonstrate that in the Ef-Art selective activation of AT-2R causes vasodilation, which opposes the vasoconstrictor action of Ang II.
KW - Glomerular hemodynamics
KW - Microperfusion CV11974
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M3 - Article
C2 - 9407460
AN - SCOPUS:0031304379
VL - 51
SP - S205-S207
JO - Kidney International, Supplement
JF - Kidney International, Supplement
SN - 0098-6577
IS - 63
ER -