FT-IR analysis of phosphorylation and laser-dephosphorylation process

Katsunori Ishii; Kaoru Yanase; Sachiko Suzuki-Yoshihashi; Masaya Yamamoto; Kunihiro Chihara; Yasuhiko Tabata; Kunio Awazu

doi:10.1117/12.591904

FT-IR analysis of phosphorylation and laser-dephosphorylation process

Katsunori Ishii, Kaoru Yanase, Sachiko Suzuki-Yoshihashi, Masaya Yamamoto, Kunihiro Chihara, Yasuhiko Tabata, Kunio Awazu

Research output: Contribution to journal › Conference article › peer-review

Abstract

Phosphorylation and dephosphorylation, which are the most remarkable posttranslational modifications, are considered to be important chemical reactions that control the activation of proteins. In regenerative medicine, it has been reported that phosphorylated proteins are instrumental in the calcification of osteoconnective tissue. We examine the phosphorylation analysis method by measuring the infrared absorption peak of phosphate group that observed at about 1070 cm^-1 (9.4 μm) with Fourier Transform Infrared Spectrometer (FT-IR). This result indicates that it is possible to identify a phosphorylation by measuring the infrared absorption peak of phosphate group observed at about 1070 cm^-1 with FT-IR method. And we examine laser-dephosphorylation using Free Electron Laser (FEL) as a novel dephosphorylation method. After irradiation of 9.4μm-FEL, infrared absorption peak of phosphate group is reduced. It is suspected that this lowering of the peak of phosphate group is the effect like dephosphorylation. These novel methods can be applied to quality control technology in regenerative medicine.

Original language	English
Article number	49
Pages (from-to)	311-316
Number of pages	6
Journal	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	5695
DOIs	https://doi.org/10.1117/12.591904
Publication status	Published - 2005
Externally published	Yes
Event	Optical Interactions with Tissue and Cells XVI - San Jose, CA, United States Duration: 2005 Jan 24 → 2005 Jan 26

Keywords

FT-IR
Free Electron Laser (FEL)
Laser-dephosphorylation
Phosphate group
Phosphorylation

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Biomaterials
Radiology Nuclear Medicine and imaging

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10.1117/12.591904

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FT-IR analysis of phosphorylation and laser-dephosphorylation process. / Ishii, Katsunori; Yanase, Kaoru; Suzuki-Yoshihashi, Sachiko; Yamamoto, Masaya; Chihara, Kunihiro; Tabata, Yasuhiko; Awazu, Kunio.

In: Progress in Biomedical Optics and Imaging - Proceedings of SPIE, Vol. 5695, 49, 2005, p. 311-316.

Research output: Contribution to journal › Conference article › peer-review

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abstract = "Phosphorylation and dephosphorylation, which are the most remarkable posttranslational modifications, are considered to be important chemical reactions that control the activation of proteins. In regenerative medicine, it has been reported that phosphorylated proteins are instrumental in the calcification of osteoconnective tissue. We examine the phosphorylation analysis method by measuring the infrared absorption peak of phosphate group that observed at about 1070 cm-1 (9.4 μm) with Fourier Transform Infrared Spectrometer (FT-IR). This result indicates that it is possible to identify a phosphorylation by measuring the infrared absorption peak of phosphate group observed at about 1070 cm-1 with FT-IR method. And we examine laser-dephosphorylation using Free Electron Laser (FEL) as a novel dephosphorylation method. After irradiation of 9.4μm-FEL, infrared absorption peak of phosphate group is reduced. It is suspected that this lowering of the peak of phosphate group is the effect like dephosphorylation. These novel methods can be applied to quality control technology in regenerative medicine.",

keywords = "FT-IR, Free Electron Laser (FEL), Laser-dephosphorylation, Phosphate group, Phosphorylation",

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AU - Ishii, Katsunori

AU - Yanase, Kaoru

AU - Suzuki-Yoshihashi, Sachiko

AU - Yamamoto, Masaya

AU - Chihara, Kunihiro

AU - Tabata, Yasuhiko

AU - Awazu, Kunio

PY - 2005

Y1 - 2005

N2 - Phosphorylation and dephosphorylation, which are the most remarkable posttranslational modifications, are considered to be important chemical reactions that control the activation of proteins. In regenerative medicine, it has been reported that phosphorylated proteins are instrumental in the calcification of osteoconnective tissue. We examine the phosphorylation analysis method by measuring the infrared absorption peak of phosphate group that observed at about 1070 cm-1 (9.4 μm) with Fourier Transform Infrared Spectrometer (FT-IR). This result indicates that it is possible to identify a phosphorylation by measuring the infrared absorption peak of phosphate group observed at about 1070 cm-1 with FT-IR method. And we examine laser-dephosphorylation using Free Electron Laser (FEL) as a novel dephosphorylation method. After irradiation of 9.4μm-FEL, infrared absorption peak of phosphate group is reduced. It is suspected that this lowering of the peak of phosphate group is the effect like dephosphorylation. These novel methods can be applied to quality control technology in regenerative medicine.

AB - Phosphorylation and dephosphorylation, which are the most remarkable posttranslational modifications, are considered to be important chemical reactions that control the activation of proteins. In regenerative medicine, it has been reported that phosphorylated proteins are instrumental in the calcification of osteoconnective tissue. We examine the phosphorylation analysis method by measuring the infrared absorption peak of phosphate group that observed at about 1070 cm-1 (9.4 μm) with Fourier Transform Infrared Spectrometer (FT-IR). This result indicates that it is possible to identify a phosphorylation by measuring the infrared absorption peak of phosphate group observed at about 1070 cm-1 with FT-IR method. And we examine laser-dephosphorylation using Free Electron Laser (FEL) as a novel dephosphorylation method. After irradiation of 9.4μm-FEL, infrared absorption peak of phosphate group is reduced. It is suspected that this lowering of the peak of phosphate group is the effect like dephosphorylation. These novel methods can be applied to quality control technology in regenerative medicine.

KW - FT-IR

KW - Free Electron Laser (FEL)

KW - Laser-dephosphorylation

KW - Phosphate group

KW - Phosphorylation

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FT-IR analysis of phosphorylation and laser-dephosphorylation process

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Keywords

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