FT-IR analysis of phosphorylation and laser-dephosphorylation process

Katsunori Ishii, Kaoru Yanase, Sachiko Suzuki-Yoshihashi, Masaya Yamamoto, Kunihiro Chihara, Yasuhiko Tabata, Kunio Awazu

Research output: Contribution to journalConference articlepeer-review

Abstract

Phosphorylation and dephosphorylation, which are the most remarkable posttranslational modifications, are considered to be important chemical reactions that control the activation of proteins. In regenerative medicine, it has been reported that phosphorylated proteins are instrumental in the calcification of osteoconnective tissue. We examine the phosphorylation analysis method by measuring the infrared absorption peak of phosphate group that observed at about 1070 cm-1 (9.4 μm) with Fourier Transform Infrared Spectrometer (FT-IR). This result indicates that it is possible to identify a phosphorylation by measuring the infrared absorption peak of phosphate group observed at about 1070 cm-1 with FT-IR method. And we examine laser-dephosphorylation using Free Electron Laser (FEL) as a novel dephosphorylation method. After irradiation of 9.4μm-FEL, infrared absorption peak of phosphate group is reduced. It is suspected that this lowering of the peak of phosphate group is the effect like dephosphorylation. These novel methods can be applied to quality control technology in regenerative medicine.

Original languageEnglish
Article number49
Pages (from-to)311-316
Number of pages6
JournalProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume5695
DOIs
Publication statusPublished - 2005
Externally publishedYes
EventOptical Interactions with Tissue and Cells XVI - San Jose, CA, United States
Duration: 2005 Jan 242005 Jan 26

Keywords

  • FT-IR
  • Free Electron Laser (FEL)
  • Laser-dephosphorylation
  • Phosphate group
  • Phosphorylation

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

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