Fruitless Represses robo1 Transcription to Shape Male-Specific Neural Morphology and Behavior in Drosophila

Hiroki Ito; Kosei Sato; Shu Kondo; Ryu Ueda; Daisuke Yamamoto

doi:10.1016/j.cub.2016.04.067

Fruitless Represses robo1 Transcription to Shape Male-Specific Neural Morphology and Behavior in Drosophila

Hiroki Ito, Kosei Sato, Shu Kondo, Ryu Ueda, Daisuke Yamamoto

Research output: Contribution to journal › Article › peer-review

34 Citations (Scopus)

Abstract

The Drosophila fruitless (fru) gene is regarded as a master regulator of the formation of male courtship circuitry, yet little is known about its molecular basis of action. We show that roundabout 1 (robo1) knockdown in females promotes formation of the male-specific neurite in sexually dimorphic mAL interneurons and that overexpression of the male-specific Fru^BM diminishes the expression of Robo1 in the fly brain. Our electrophoretic mobility shift and reporter assays identify the 42-bp segment encompassing the palindrome sequence T T C G C T G C G C C G T G A A in the 5′ UTR of robo1 exon1 as the Fru^BM-responsive element. We find that ∼10-bp deletions in the palindrome sequence induce a loss of the male-specific neurite and disrupt male courtship patterns. This study paves the way for a thorough understanding of the mechanism whereby Fru proteins orchestrate transcription for the formation of courtship circuitry.

Original language	English
Pages (from-to)	1532-1542
Number of pages	11
Journal	Current Biology
Volume	26
Issue number	12
DOIs	https://doi.org/10.1016/j.cub.2016.04.067
Publication status	Published - 2016

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Agricultural and Biological Sciences(all)

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10.1016/j.cub.2016.04.067

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title = "Fruitless Represses robo1 Transcription to Shape Male-Specific Neural Morphology and Behavior in Drosophila",

abstract = "The Drosophila fruitless (fru) gene is regarded as a master regulator of the formation of male courtship circuitry, yet little is known about its molecular basis of action. We show that roundabout 1 (robo1) knockdown in females promotes formation of the male-specific neurite in sexually dimorphic mAL interneurons and that overexpression of the male-specific FruBM diminishes the expression of Robo1 in the fly brain. Our electrophoretic mobility shift and reporter assays identify the 42-bp segment encompassing the palindrome sequence T T C G C T G C G C C G T G A A in the 5′ UTR of robo1 exon1 as the FruBM-responsive element. We find that ∼10-bp deletions in the palindrome sequence induce a loss of the male-specific neurite and disrupt male courtship patterns. This study paves the way for a thorough understanding of the mechanism whereby Fru proteins orchestrate transcription for the formation of courtship circuitry.",

author = "Hiroki Ito and Kosei Sato and Shu Kondo and Ryu Ueda and Daisuke Yamamoto",

note = "Funding Information: We are grateful to the Bloomington Drosophila Stock Center and Vienna Drosophila Resource Center for fly stocks; the Developmental Studies Hybridoma Bank for antibodies; the Drosophila Genomics Resource Center for a genomic clone; S. Goodwin, K.-I. Kimura, I. Salecker, and K. Scott for flies; and M. Suyama and A. Utsumi for secretarial assistance. This work is supported by Grants-in-Aid for Scientific Research (25430001 to H.I.; 15K14306, 26113702, 26114502, 23220007, and 25132702 to D.Y.; and 24700309 to K.S.) from MEXT and from the Takeda Science Foundation to D.Y. and K.S. Publisher Copyright: {\textcopyright} 2016 Elsevier Ltd",

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AU - Yamamoto, Daisuke

N1 - Funding Information: We are grateful to the Bloomington Drosophila Stock Center and Vienna Drosophila Resource Center for fly stocks; the Developmental Studies Hybridoma Bank for antibodies; the Drosophila Genomics Resource Center for a genomic clone; S. Goodwin, K.-I. Kimura, I. Salecker, and K. Scott for flies; and M. Suyama and A. Utsumi for secretarial assistance. This work is supported by Grants-in-Aid for Scientific Research (25430001 to H.I.; 15K14306, 26113702, 26114502, 23220007, and 25132702 to D.Y.; and 24700309 to K.S.) from MEXT and from the Takeda Science Foundation to D.Y. and K.S. Publisher Copyright: © 2016 Elsevier Ltd

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N2 - The Drosophila fruitless (fru) gene is regarded as a master regulator of the formation of male courtship circuitry, yet little is known about its molecular basis of action. We show that roundabout 1 (robo1) knockdown in females promotes formation of the male-specific neurite in sexually dimorphic mAL interneurons and that overexpression of the male-specific FruBM diminishes the expression of Robo1 in the fly brain. Our electrophoretic mobility shift and reporter assays identify the 42-bp segment encompassing the palindrome sequence T T C G C T G C G C C G T G A A in the 5′ UTR of robo1 exon1 as the FruBM-responsive element. We find that ∼10-bp deletions in the palindrome sequence induce a loss of the male-specific neurite and disrupt male courtship patterns. This study paves the way for a thorough understanding of the mechanism whereby Fru proteins orchestrate transcription for the formation of courtship circuitry.

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Fruitless Represses robo1 Transcription to Shape Male-Specific Neural Morphology and Behavior in Drosophila

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