Fruitless Represses robo1 Transcription to Shape Male-Specific Neural Morphology and Behavior in Drosophila

Hiroki Ito, Kosei Sato, Shu Kondo, Ryu Ueda, Daisuke Yamamoto

    Research output: Contribution to journalArticlepeer-review

    29 Citations (Scopus)

    Abstract

    The Drosophila fruitless (fru) gene is regarded as a master regulator of the formation of male courtship circuitry, yet little is known about its molecular basis of action. We show that roundabout 1 (robo1) knockdown in females promotes formation of the male-specific neurite in sexually dimorphic mAL interneurons and that overexpression of the male-specific FruBM diminishes the expression of Robo1 in the fly brain. Our electrophoretic mobility shift and reporter assays identify the 42-bp segment encompassing the palindrome sequence T T C G C T G C G C C G T G A A in the 5′ UTR of robo1 exon1 as the FruBM-responsive element. We find that ∼10-bp deletions in the palindrome sequence induce a loss of the male-specific neurite and disrupt male courtship patterns. This study paves the way for a thorough understanding of the mechanism whereby Fru proteins orchestrate transcription for the formation of courtship circuitry.

    Original languageEnglish
    Pages (from-to)1532-1542
    Number of pages11
    JournalCurrent Biology
    Volume26
    Issue number12
    DOIs
    Publication statusPublished - 2016

    ASJC Scopus subject areas

    • Biochemistry, Genetics and Molecular Biology(all)
    • Agricultural and Biological Sciences(all)

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