Fruit-specific V-ATPase suppression in antisense-transgenic tomato reduces fruit growth and seed formation

Tsuyoshi Amemiya, Yoshinori Kanayama, Shohei Yamaki, Kunio Yamada, Katsuhiro Shiratake

Research output: Contribution to journalArticlepeer-review

37 Citations (Scopus)

Abstract

The vacuole is a large, multifunctional organelle related to the processes of cell expansion, solute accumulation, regulation of cytoplasmic ion concentrations, pH homeostasis and osmoregulation, which are directly or indirectly achieved by vacuolar H+-pumps: vacuolar H +-ATPase (V-ATPase; EC 3.6.1.3) and vacuolar H+- pyrophosphatase (V-PPase; EC 3.6.1.1). In this study, we produced antisense-transgenic tomatoes (Lycopersicon esculentum L.) of the V-ATPase A subunit, which is under the control of the fruit-specific 2A11 promoter. One β-glucuronidase (GUS)-transgenic line (GUS control) and seven A subunit antisense-transgenic lines were obtained. The amount of V-ATPase A subunit mRNA in fruit decreased in all antisense-transgenic lines, but in leaves showed no difference compared with the GUS control line and the nontransformant, suggesting that suppression of the V-ATPase A subunit by a 2A11 promoter is limited to fruit. The antisense-transgenic plants had smaller fruits compared with the GUS control line and the nontransformant. Surprisingly, fruits from the antisense-transgenic plants, except the fruit that still had relatively high expression of A subunit mRNA, had few seeds. Sucrose concentration in fruits from the antisense-transgenic plants increased, but glucose and fructose concentrations did not change. These results show the importance of V-ATPase, not only in fruit growth, but also in seed formation and in sugar composition of tomato fruit.

Original languageEnglish
Pages (from-to)1272-1280
Number of pages9
JournalPlanta
Volume223
Issue number6
DOIs
Publication statusPublished - 2006 May

Keywords

  • Fruit growth
  • Gene suppression
  • Lycopersicon
  • Seed formation
  • Tomato
  • Vacuolar H-ATPase

ASJC Scopus subject areas

  • Genetics
  • Plant Science

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