FRET-based intracellular investigation of nanoprodrugs toward highly efficient anticancer drug delivery

Farsai Taemaitree, Beatrice Fortuni, Yoshitaka Koseki, Eduard Fron, Susana Rocha, Johan Hofkens, Hiroshi Uji-I, Tomoko Inose, Hitoshi Kasai

    Research output: Contribution to journalArticlepeer-review

    9 Citations (Scopus)

    Abstract

    In order to overcome unpredictable side-effects and increased cytotoxicity of conventional carrier-based anticancer drug delivery systems, several systems that consist exclusively of the pure drug (or prodrug) have been proposed. The behavior and dynamics of these systems after entering cancer cells are, however, still unknown, hindering their progress towards in vivo and clinical applications. Here, we report a comprehensive in cellulo study of carrier-free SN-38 nanoprodrugs (NPDs), previously developed by our group. The work shows the intracellular uptake, localization, and degradation of the NPDs via FRET microscopy. Accordingly, new FRET-NPDs were chemically synthesized and characterized. Prodrug to drug conversion and therapeutic efficiency were also validated. Our work provides crucial information for the application of NPDs as drug delivery systems and demonstrates their outstanding potential as next-generation anticancer nanomedicines.

    Original languageEnglish
    Pages (from-to)16710-16715
    Number of pages6
    JournalNanoscale
    Volume12
    Issue number32
    DOIs
    Publication statusPublished - 2020 Aug 28

    ASJC Scopus subject areas

    • Materials Science(all)

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