Frequent overexpression, but not activation by point mutation, of ras genes in primary human gastric cancers

Koichi Fujita, Noriaki Ohuchi, Tsuneyoshi Yao, Makoto Okumura, Yukio Fukushima, Yuzuru Kanakura, Yukihiko Kitamura, Jun Fujita

Research output: Contribution to journalArticlepeer-review

45 Citations (Scopus)

Abstract

To define the extent of involvement of ras oncogenes in human gastric cancers, we surveyed for the presence of ras oncogenes, activated by either point mutations within their coding sequences or overexpression of ras protein p21, by the combined use of several analytic techniques. Primary gastric cancers were first analyzed by deoxyribonucleic acid transfection assay using NIH/3T3 cells as recipients and by restriction enzyme analysis, which detects point mutations at codon 12 of the H-ras gene. None of seven tumors analyzed scored as positive. Furthermore, none of them had ras p21 with altered electrophoretic mobility on immunoprecipitation and Western blotting, confirming the absence of ras oncogenes activated by point mutations in these tumors. However, in 6 of 7 tumors, the amounts of p21 exceeded that in human placenta. Amplification of the K-ras gene was found in 1 of 11 (including the 7 described above) gastric cancers. Immunohistochemical analysis of ras p21 expression in these 11 tumors was then carried out using the anti-ras p21 monoclonal antibody RAP-5. All cancers showed more reactivity with RAP-5 than did normal mucosa adjacent to the cancers, indicating increased expression of ras p21. These results indicated that transformation of the stomach mucosa from the normal to the malignant phenotype is rarely associated with activation of ras genes by point mutations, but is frequently associated with enhanced expression of ras p21.

Original languageEnglish
Pages (from-to)1339-1345
Number of pages7
JournalGastroenterology
Volume93
Issue number6
DOIs
Publication statusPublished - 1987 Dec

ASJC Scopus subject areas

  • Hepatology
  • Gastroenterology

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