Frequent gain of copy number on the long arm of chromosome 20 in human pancreatic adenocarcinoma

Shinichi Fukushige, Frederic M. Waldman, Mitsuhiro Kimura, Tadayoshi Abe, Toru Furukawa, Makoto Sunamura, Masao Kobari, Akira Horii

Research output: Contribution to journalArticle

118 Citations (Scopus)

Abstract

We have used comparative genomic hybridization (CGH) to survey genomic regions with aberrant copy numbers of DNA sequences in pancreatic adenocarcinoma. In 12 cell lines and 6 primary tumors from 18 patients with pancreatic adenocarcinomas, highly frequent losses (>60%) were observed on chromosome arms 6q, 9p, and 18q and the Y chromosome. Moderately frequent losses (40-60%) were observed on chromosome arms 3p, 4q, 8p, and 21q. Interestingly, these samples showed extremely high frequencies of increases in copy numbers of DNA sequences on the long arm of chromosome 20 (15/18, 83%). We further analyzed five cell lines by fluorescence in situ hybridization (FISH) with probes on chromosome 20 to define the increase in copy number more accurately, and we found that 20q was increased to between 5 and 8 copies per cell. These results suggest the existence of an oncogene or oncogenes on 20q that play a role in the development and/or the progression of pancreatic carcinogenesis.

Original languageEnglish
Pages (from-to)161-169
Number of pages9
JournalGenes Chromosomes and Cancer
Volume19
Issue number3
DOIs
Publication statusPublished - 1997 Jul 15

ASJC Scopus subject areas

  • Genetics
  • Cancer Research

Fingerprint Dive into the research topics of 'Frequent gain of copy number on the long arm of chromosome 20 in human pancreatic adenocarcinoma'. Together they form a unique fingerprint.

  • Cite this