TY - JOUR
T1 - Frequent deletions of material from chromosome arm 1p in oligodendroglial tumors revealed by double‐target fluorescence in situ hybridization and microsatellite analysis
AU - Hashimoto, Naoya
AU - Ichikawa, Daisuke
AU - Arakawa, Yoshiki
AU - Date, Kousei
AU - Ueda, Satoshi
AU - Nakagawa, Yoshio
AU - Horii, Akira
AU - Nakamura, Yusuke
AU - Abe, Tatsuo
AU - Inazawa, Johji
PY - 1995/12
Y1 - 1995/12
N2 - We undertook a cytogenetic analysis of 29 human brain tumors using double‐target fluorescence in situ hybridization (FISH) and focusing on chromosome arm 1p. One or more tumor suppressor genes in this arm have been suggested to be important in a variety of neuroectodermal tumors. The series included 9 oligodendrogliomas, 4 mixed gliomas, 10 astrocytomas, 4 glioblastomas, and 2 central neurocytomas. We hybridized pericentromeric (1q12) and subtelomeric (1p36) DNA probes to cell nuclei prepared from paraffin‐embedded tissues and observed a strikingly high incidence of deletion of at least part of 1p in oligodendrogliomas (100%) and mixed gliomas (75%). The results of the FISH analyses were confirmed by demonstration of loss of heterozygosity for a microsatellite polymorphism in 10 of the 29 tumors. As well as supporting the feasibility of FISH for detecting allelic deletions in chromosomes from paraffin‐embedded tumor samples, the alteration of 1p reported here will contribute to an understanding of the molecular genetic events in oligodendroglial tumor development.
AB - We undertook a cytogenetic analysis of 29 human brain tumors using double‐target fluorescence in situ hybridization (FISH) and focusing on chromosome arm 1p. One or more tumor suppressor genes in this arm have been suggested to be important in a variety of neuroectodermal tumors. The series included 9 oligodendrogliomas, 4 mixed gliomas, 10 astrocytomas, 4 glioblastomas, and 2 central neurocytomas. We hybridized pericentromeric (1q12) and subtelomeric (1p36) DNA probes to cell nuclei prepared from paraffin‐embedded tissues and observed a strikingly high incidence of deletion of at least part of 1p in oligodendrogliomas (100%) and mixed gliomas (75%). The results of the FISH analyses were confirmed by demonstration of loss of heterozygosity for a microsatellite polymorphism in 10 of the 29 tumors. As well as supporting the feasibility of FISH for detecting allelic deletions in chromosomes from paraffin‐embedded tumor samples, the alteration of 1p reported here will contribute to an understanding of the molecular genetic events in oligodendroglial tumor development.
UR - http://www.scopus.com/inward/record.url?scp=0028783480&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028783480&partnerID=8YFLogxK
U2 - 10.1002/gcc.2870140408
DO - 10.1002/gcc.2870140408
M3 - Article
C2 - 8605118
AN - SCOPUS:0028783480
VL - 14
SP - 295
EP - 300
JO - Genes Chromosomes and Cancer
JF - Genes Chromosomes and Cancer
SN - 1045-2257
IS - 4
ER -