Frequency and pattern of transposition of the maize transposable element Ds in transgenic rice plants

Yuko Nakagawa, Chiyoko Machida, Yasunori Machida, Kinya Toriyama

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Two kinds of T-DNA constructs, I-RS/dAc-I-RS and Hm(R)Ds, carrying a non-autonomous transposable element of Ac of maize were introduced into rice plants by Agrobacterium-mediated gene transfer. Six transgenic rice plants identified as containing a single copy of the element were crossed with two transgenic rice plants carrying a gene for Ac transposase under the control of the cauliflower mosaic virus 35S promoter. In F2 progenies, excision of the element was detected by PCR analysis and re-integration of the element was investigated by Southern blot analysis. The frequency of the excision of the element was found to vary from 0 to 70% depending on the crossing combination. The frequency of the number of individual transposition events out of the total number of F2 plants with germinal excision was 44% in one crossing combination and 38% in the other. In the most efficient case, 10 plants with independent transposition were obtained out of the 49 F2 plants tested. Linkage analysis of the empty donor site and the transposed Ds-insertion site in F3 plants demonstrated that one of five Ds-insertion sites was not linked to the empty donor site. The transgenic rice obtained in this study can he used for functional genomics of rice.

Original languageEnglish
Pages (from-to)733-742
Number of pages10
JournalPlant and Cell Physiology
Volume41
Issue number6
DOIs
Publication statusPublished - 2000 Jan 1

Keywords

  • Agrobacterium-mediated gene transfer
  • Ds transposition
  • Oryza sativa L.
  • Transposon Ac/Ds

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

Fingerprint Dive into the research topics of 'Frequency and pattern of transposition of the maize transposable element Ds in transgenic rice plants'. Together they form a unique fingerprint.

  • Cite this