Free-standing lipid bilayers in silicon chips-membrane stabilization based on microfabricated apertures with a nanometer-scale smoothness

Ayumi Hirano-Iwata, Kouji Aoto, Azusa Oshima, Tasuku Taira, Ryo Taro Yamaguchi, Yasuo Kimura, Michio Niwano

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

In the present study, we propose a method for preparing stable free-standing bilayer lipid membranes (BLMs). The BLMs were prepared in a microfabricated aperture with a smoothly tapered edge, which was prepared in a nanometerthick Si3N4 septum by the wet etching method. Owing to this structure, the stress on lipid, bilayers at the contact with the septum was minimized, leading to remarkable membrane stability. The BLMs were not broken by applying a constant voltage of ±1V. The membrane lifetime was 15-45 h with and without an incorporated gramicidin channel. Gramicidin single-channel currents were recorded from the same BLM preparation when the aqueous solutions surrounding the BLM were repeatedly exchanged, demonstrating the tolerance of the present BLM to repetitive solution exchanges. Such stable membranes enable analysis of channel functions under various solution conditions from the same BLM, which will open up a variety of applications including a high throughput drug screening for ion channels.

Original languageEnglish
Pages (from-to)1949-1952
Number of pages4
JournalLangmuir
Volume26
Issue number3
DOIs
Publication statusPublished - 2010 Feb 2

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

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