Free radical scavenger edaravone suppresses X-ray-induced apoptosis through p53 inhibition in MOLT-4 cells

Nakashi Sasano, Atsushi Enomoto, Yoshio Hosoi, Yosuke Katsumura, Yoshihisa Matsumoto, Kenshiro Shiraishi, Kiyoshi Miyagawa, Hiroshi Igaki, Keiichi Nakagawa

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

Edaravone, a clinical drug used widely for the treatment of acute cerebral infarction, is reported to scavenge free radicals. In the present study, we investigated the radioprotective effect of edaravone on X-ray-induced apoptosis in MOLT-4 cells. Apoptosis was determined by the dye exclusion test, Annexin V binding assay, cleavage of caspase, and DNA fragmentation. We found that edaravone significantly suppressed the X-ray-induced apoptosis. The amount of intracellular ROS production was determined by the chloromethyl-2′, 7′-dichlorodihydro-fluorescein diacetate system. We found that the intracellular ROS production by X-irradiation was completely suppressed by the addition of edaravone. The accumulation and phosphorylation of p53 and the expression of p21 WAF1, a target protein of p53, which were induced by X-irradiation, were also suppressed by adding edaravone. We conclude that the free radical scavenger edaravone suppresses X-ray-induced apoptosis in MOLT-4 cells by inhibiting p53.

Original languageEnglish
Pages (from-to)495-503
Number of pages9
JournalJournal of radiation research
Volume48
Issue number6
DOIs
Publication statusPublished - 2007
Externally publishedYes

Keywords

  • Edaravone
  • Free radical scavenger
  • MOLT-4 cells
  • Radioprotective effect
  • X-ray-induced apoptosis

ASJC Scopus subject areas

  • Radiation
  • Radiology Nuclear Medicine and imaging
  • Health, Toxicology and Mutagenesis

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