Free radical generation from heterocyclic amines by cytochrome b5 reductase in the presence of NADH

Hiroshi Maeda, Tomohiro Sawa, Toshitugu Yubisui, Takaaki Akaike

Research output: Contribution to journalArticlepeer-review

34 Citations (Scopus)

Abstract

We previously reported findings that NADPH/cytochrome P450 reductase can generate superoxide anion radical (O2(.-)) from heterocyclic amines (HCA) and from many anticancer agents in vitro. Here we present more evidence in which O2(.-) is generated when recombinant human cytochrome b5 reductase (rh-Cytb5Rd) was incubated with HCAs such as IQ and MeIQ in the presence of NADH in vitro. This indicates that free radical generation by rh-Cytb5Rd in the presence of HCA may add new insight into the damage of DNA in addition to the previously known mechanism: interaction of activated HCA-intermediates to form DNA adduct. Copyright (C) 1999 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)117-121
Number of pages5
JournalCancer Letters
Volume143
Issue number2
DOIs
Publication statusPublished - 1999 Sep 1
Externally publishedYes

Keywords

  • Cytochrome b5 reductase
  • DNA damage
  • Electron spin resonance
  • Heterocyclic amines
  • Superoxide generation

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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