Fractionation of nitrogen isotopes by glutamine synthetase isolated from spinach leaves

Tadakatsu Yoneyama; Kazunari Kamachi; Tomoyuki Yamaya; Tadahiko Mae

Fractionation of nitrogen isotopes by glutamine synthetase isolated from spinach leaves

Tadakatsu Yoneyama, Kazunari Kamachi, Tomoyuki Yamaya, Tadahiko Mae

Division for Interdisciplinary Advanced Research and Education

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52 Citations (Scopus)

Abstract

The isotopic fractionation of nitrogen in the reaction in vitro of glutamine synthetase isolated from spinach (Spinacia oleracea L.) leaves was calculated from the changes in natural ¹⁵N abundance (δ¹⁵N value) of ammonia and amide of glutamine during the incubation. The observed isotope fractionation under our conditions was -16.5±1.5%.

Original language	English
Pages (from-to)	489-491
Number of pages	3
Journal	Plant and Cell Physiology
Volume	34
Issue number	3
Publication status	Published - 1993 Apr 1

Keywords

Glutamine synthetase
Isotope fractionation
Nitrogen isotopes
Spinach (Spinacia oleracea L.).

ASJC Scopus subject areas

Physiology
Plant Science
Cell Biology

Cite this

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title = "Fractionation of nitrogen isotopes by glutamine synthetase isolated from spinach leaves",

abstract = "The isotopic fractionation of nitrogen in the reaction in vitro of glutamine synthetase isolated from spinach (Spinacia oleracea L.) leaves was calculated from the changes in natural 15N abundance (δ15N value) of ammonia and amide of glutamine during the incubation. The observed isotope fractionation under our conditions was -16.5±1.5%.",

keywords = "Glutamine synthetase, Isotope fractionation, Nitrogen isotopes, Spinach (Spinacia oleracea L.).",

author = "Tadakatsu Yoneyama and Kazunari Kamachi and Tomoyuki Yamaya and Tadahiko Mae",

year = "1993",

month = apr,

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pages = "489--491",

journal = "Plant and Cell Physiology",

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T1 - Fractionation of nitrogen isotopes by glutamine synthetase isolated from spinach leaves

AU - Yoneyama, Tadakatsu

AU - Kamachi, Kazunari

AU - Yamaya, Tomoyuki

AU - Mae, Tadahiko

PY - 1993/4/1

Y1 - 1993/4/1

N2 - The isotopic fractionation of nitrogen in the reaction in vitro of glutamine synthetase isolated from spinach (Spinacia oleracea L.) leaves was calculated from the changes in natural 15N abundance (δ15N value) of ammonia and amide of glutamine during the incubation. The observed isotope fractionation under our conditions was -16.5±1.5%.

AB - The isotopic fractionation of nitrogen in the reaction in vitro of glutamine synthetase isolated from spinach (Spinacia oleracea L.) leaves was calculated from the changes in natural 15N abundance (δ15N value) of ammonia and amide of glutamine during the incubation. The observed isotope fractionation under our conditions was -16.5±1.5%.

KW - Glutamine synthetase

KW - Isotope fractionation

KW - Nitrogen isotopes

KW - Spinach (Spinacia oleracea L.).

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M3 - Article

AN - SCOPUS:0000937967

VL - 34

SP - 489

EP - 491

JO - Plant and Cell Physiology

JF - Plant and Cell Physiology

SN - 0032-0781

IS - 3

ER -

Fractionation of nitrogen isotopes by glutamine synthetase isolated from spinach leaves

Abstract

Keywords

ASJC Scopus subject areas

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