Fractionation of nitrogen isotopes by glutamine synthetase isolated from spinach leaves

Tadakatsu Yoneyama, Kazunari Kamachi, Tomoyuki Yamaya, Tadahiko Mae

    Research output: Contribution to journalArticlepeer-review

    49 Citations (Scopus)

    Abstract

    The isotopic fractionation of nitrogen in the reaction in vitro of glutamine synthetase isolated from spinach (Spinacia oleracea L.) leaves was calculated from the changes in natural 15N abundance (δ15N value) of ammonia and amide of glutamine during the incubation. The observed isotope fractionation under our conditions was -16.5±1.5%.

    Original languageEnglish
    Pages (from-to)489-491
    Number of pages3
    JournalPlant and Cell Physiology
    Volume34
    Issue number3
    Publication statusPublished - 1993 Apr 1

    Keywords

    • Glutamine synthetase
    • Isotope fractionation
    • Nitrogen isotopes
    • Spinach (Spinacia oleracea L.).

    ASJC Scopus subject areas

    • Physiology
    • Plant Science
    • Cell Biology

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