Fractionation of nitrogen isotopes by glutamine synthetase isolated from spinach leaves

Tadakatsu Yoneyama, Kazunari Kamachi, Tomoyuki Yamaya, Tadahiko Mae

Research output: Contribution to journalArticlepeer-review

52 Citations (Scopus)

Abstract

The isotopic fractionation of nitrogen in the reaction in vitro of glutamine synthetase isolated from spinach (Spinacia oleracea L.) leaves was calculated from the changes in natural 15N abundance (δ15N value) of ammonia and amide of glutamine during the incubation. The observed isotope fractionation under our conditions was -16.5±1.5%.

Original languageEnglish
Pages (from-to)489-491
Number of pages3
JournalPlant and Cell Physiology
Volume34
Issue number3
Publication statusPublished - 1993 Apr 1

Keywords

  • Glutamine synthetase
  • Isotope fractionation
  • Nitrogen isotopes
  • Spinach (Spinacia oleracea L.).

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

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