Four new human renal cell carcinoma cell lines expressing globo-series gangliosides

Makoto Satoh; Fatemeh Moosavi Nejad; Osamu Nakano; Akihiro Ito; Sadafumi Kawamura; Chikara Ohyama; Seiichi Saito; Seiichi Orikasa

doi:10.1620/tjem.189.95

Four new human renal cell carcinoma cell lines expressing globo-series gangliosides

Makoto Satoh, Fatemeh Moosavi Nejad, Osamu Nakano, Akihiro Ito, Sadafumi Kawamura, Chikara Ohyama, Seiichi Saito, Seiichi Orikasa

MED - Medical Sciences

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

Clinicopathological studies revealed that monosialosyl galactosyl globoside (MSGG) and disialosyl galactosyl globoside (DSGG) expressed by renal cell carcinoma (RCC) are one of the biochemical indicator related to the metastatic potential. The present study examines the characteristics of four new human RCC cell lines and compares the expression of MSGG and DSGG among them using TLC immunostaining and flow cytometry. TOS-1 and TOS-2 were derived from metastatic subcutaneous tissues. TOS-3 and TOS-3LN were derived from the primary lesion and from metastatic lymph nodes respectively. Monolayer culture, light microscopy and electron microscopy of these cells showed that these cell lines were derived from RCC. TLC immunostaining and flow cytometric analysis revealed increased levels of MSGG in TOS-2 and TOS-3LN, and increased DSGG in TOS-1 and TOS-3LN. These cell lines would be useful for functional studies of globo-series ganglioside expressed by RCC.

Original language	English
Pages (from-to)	95-105
Number of pages	11
Journal	Tohoku Journal of Experimental Medicine
Volume	189
Issue number	2
DOIs	https://doi.org/10.1620/tjem.189.95
Publication status	Published - 1999 Oct

Keywords

Established cell lines
Globo-series ganglioside
Renal cell carcinoma

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

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Four new human renal cell carcinoma cell lines expressing globo-series gangliosides. / Satoh, Makoto; Nejad, Fatemeh Moosavi; Nakano, Osamu; Ito, Akihiro; Kawamura, Sadafumi; Ohyama, Chikara; Saito, Seiichi; Orikasa, Seiichi.

In: Tohoku Journal of Experimental Medicine, Vol. 189, No. 2, 10.1999, p. 95-105.

Research output: Contribution to journal › Article › peer-review

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abstract = "Clinicopathological studies revealed that monosialosyl galactosyl globoside (MSGG) and disialosyl galactosyl globoside (DSGG) expressed by renal cell carcinoma (RCC) are one of the biochemical indicator related to the metastatic potential. The present study examines the characteristics of four new human RCC cell lines and compares the expression of MSGG and DSGG among them using TLC immunostaining and flow cytometry. TOS-1 and TOS-2 were derived from metastatic subcutaneous tissues. TOS-3 and TOS-3LN were derived from the primary lesion and from metastatic lymph nodes respectively. Monolayer culture, light microscopy and electron microscopy of these cells showed that these cell lines were derived from RCC. TLC immunostaining and flow cytometric analysis revealed increased levels of MSGG in TOS-2 and TOS-3LN, and increased DSGG in TOS-1 and TOS-3LN. These cell lines would be useful for functional studies of globo-series ganglioside expressed by RCC.",

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AU - Nejad, Fatemeh Moosavi

AU - Nakano, Osamu

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AU - Kawamura, Sadafumi

AU - Ohyama, Chikara

AU - Saito, Seiichi

AU - Orikasa, Seiichi

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N2 - Clinicopathological studies revealed that monosialosyl galactosyl globoside (MSGG) and disialosyl galactosyl globoside (DSGG) expressed by renal cell carcinoma (RCC) are one of the biochemical indicator related to the metastatic potential. The present study examines the characteristics of four new human RCC cell lines and compares the expression of MSGG and DSGG among them using TLC immunostaining and flow cytometry. TOS-1 and TOS-2 were derived from metastatic subcutaneous tissues. TOS-3 and TOS-3LN were derived from the primary lesion and from metastatic lymph nodes respectively. Monolayer culture, light microscopy and electron microscopy of these cells showed that these cell lines were derived from RCC. TLC immunostaining and flow cytometric analysis revealed increased levels of MSGG in TOS-2 and TOS-3LN, and increased DSGG in TOS-1 and TOS-3LN. These cell lines would be useful for functional studies of globo-series ganglioside expressed by RCC.

AB - Clinicopathological studies revealed that monosialosyl galactosyl globoside (MSGG) and disialosyl galactosyl globoside (DSGG) expressed by renal cell carcinoma (RCC) are one of the biochemical indicator related to the metastatic potential. The present study examines the characteristics of four new human RCC cell lines and compares the expression of MSGG and DSGG among them using TLC immunostaining and flow cytometry. TOS-1 and TOS-2 were derived from metastatic subcutaneous tissues. TOS-3 and TOS-3LN were derived from the primary lesion and from metastatic lymph nodes respectively. Monolayer culture, light microscopy and electron microscopy of these cells showed that these cell lines were derived from RCC. TLC immunostaining and flow cytometric analysis revealed increased levels of MSGG in TOS-2 and TOS-3LN, and increased DSGG in TOS-1 and TOS-3LN. These cell lines would be useful for functional studies of globo-series ganglioside expressed by RCC.

KW - Established cell lines

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Four new human renal cell carcinoma cell lines expressing globo-series gangliosides

Abstract

Keywords

ASJC Scopus subject areas

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