Formazan derivatives as the precolumn derivatization reagents in a coupled high-performance liquid chromatographic-spectrophotometric system for trace metal determination

Hitoshi Hoshino, Kouji Nakano, Takao Yotsuyanagi

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

As precolumn chelating reagents for use in high-performance liquid chromatographic (HPLC) determinations of ultratrace metal ions, several kinds of 3-phenyl- and 3-cyanoformazans were examined and the reagent structure is discussed in connection with the HPLC selectivity for metal ions. These zincon-type formazans, functioning as quadridentate ligands, provide excellent ability in the precolumn derivatization scheme for spectrophotometric detection. The coplanarity and the chelate-cage effect of the reagent skeleton are probably responsible for the unique selectivity for CuII and ZnII ions, respectively. The formazans possessing oxygen donors give a good resolution of trivalent metal ions, which seems to be ascribable to the hard-base character produced by the oxygen atoms. Detectability for the NiII ion is only shown with a reagent that provides an α-diimine-like coordination environment consisting of a four-N donor assembly. The limits of detection for most of the metal ions tested are down to the nanomoles per litre level (sub-picomole amounts). Highly sensitive HPLC methods for metal ions at sub-ppb to ppb (10-9 g/g) levels are suggested.

Original languageEnglish
Pages (from-to)603-610
Number of pages8
JournalJournal of Chromatography A
Volume515
Issue numberC
DOIs
Publication statusPublished - 1990 Aug 31

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

Fingerprint Dive into the research topics of 'Formazan derivatives as the precolumn derivatization reagents in a coupled high-performance liquid chromatographic-spectrophotometric system for trace metal determination'. Together they form a unique fingerprint.

Cite this