Formation of lipid droplets induced by 2,3-dihydrogeranylgeranoic acid distinct from geranylgeranoic acid

Yuichi Kodaira, Takeru Kusumoto, Takeshi Takahashi, Yoshihiro Matsumura, Yukino Miyagi, Kyoko Okamoto, Yoshihiro Shidoji, Hiroshi Sagami

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Geranylgeranoic acid (GGA) and 2,3-dihydrogeranylgeranoic acid (2,3-diGGA) are geranylgeraniol-derived metabolites (Kodaira et al. (2002) J Biochem 132: 327-334). In the present study, we examined the effects of these acids on HL-60 cells. The cells were differentiated into neutrophils by GGA stimulation like retinoic acid stimulation. In the case of cells stimulated with 2,3-diGGA, neutrophils were not detected, but the formation of lipid droplets was induced. On the other hand, when the cells were cultured in the presence of 0.1% FBS instead of 10% FBS, apoptotic cells were induced not only by GGA stimulation but also with 2,3-diGGA. In the latter case, when the cells were cultured in the co-presence of a caspase-3 inhibitor (Ac-DMQD-CHO), the lipid droplets formation was observed in the cells. These results suggest that GGA and 2,3-diGGA are extremely different from each other with respect to their effects on HL-60 cells.

Original languageEnglish
Pages (from-to)777-782
Number of pages6
JournalActa biochimica Polonica
Volume54
Issue number4
DOIs
Publication statusPublished - 2007

Keywords

  • 2,3-dihydrogeranylgeranoic acid
  • Geranylgeranoic acid
  • HL-60
  • Lipid droplets
  • Neutrophils

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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