Formation of hydrogen-producing granules and microbial community analysis in a UASB reactor

Yan Yan Ning, Shao Feng Wang, Da Wei Jin, Hideki Harada, Xian Yang Shi

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)


The effect of substrate concentration on continuous H2 production from a sucrose-rich synthetic wastewater was investigated in this work. The maximum H2 production rate of 2.89 m3 H2/m3/d achieved at 7000 mg COD/L while the peak ethanol concentration of 2840 mg/L was observed at 5000 mg COD/L. The H2-producing granules with ethanol-type fermentation were successfully cultivated in an upflow anaerobic sludge blanket (UASB) reactor. Denaturing gradient gel electrophoresis analysis demonstrates that the substrate concentration had a considerable effect on the microbial community. After the mature H2-producing granules were formed, the microbial community structure in the UASB reactor tends to stabilize. The dominant bacterium in the mature H2-producing granules were composed of Janthinobacterium sp. WPCB148, Clostridium sp. HPB-4, Variovorax paradoxus strain SJ100, Variovorax sp. CN3b and Uncultured bacterium clone C2.

Original languageEnglish
Pages (from-to)12-17
Number of pages6
JournalRenewable Energy
Publication statusPublished - 2013 May


  • Granule
  • Hydrogen production
  • Upflow anaerobic sludge blanket (UASB) reactor

ASJC Scopus subject areas

  • Renewable Energy, Sustainability and the Environment


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