Formation of abasic sites in DNA by t-butyl peroxyl radicals: Implication for potent genotoxicity of lipid peroxyl radicals

Ayako Kanazawa, Tomohiro Sawa, Takaaki Akaik, Hiroshi Maeda

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

We investigated abasic site formation in calf thymus DNA after exposure to a model compound of lipid-derived peroxyl radical that was generated by the reaction of tert-butyl hydroperoxide (t-BuOOH) with hemoglobin. Abasic site density in DNA was quantified by use of an enzyme-linked immunosorvent assay-like assay. In the presence of 10 mM t-BuOOH and 12.5 or 25 μM hemoglobin, 0.6-1.0 abasic sites/104 nucleotides were formed. However, abasic sites were not detected after replacing hemoglobin with nonheme iron, e.g. EDTA/Fe2+, which initiates the production of alkyl and alkoxyl radicals. Therefore, the present results suggest that lipid peroxyl radicals may have a genotoxic potential through unique reactions, including depurination and depyrimidination, which lead to DNA strand breakage. Copyright (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)51-55
Number of pages5
JournalCancer Letters
Volume156
Issue number1
DOIs
Publication statusPublished - 2000 Aug 1
Externally publishedYes

Keywords

  • Abasic site
  • Alkyl peroxyl radical
  • DNA modification

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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