Formation and flash-dependent oscillation of the S2-state multiline EPR signal in an oxygen-evolving Photosystem-II preparation lacking the three extrinsic proteins in the oxygen-evolving system

Stenbjörn Styring; Mitsue Miyao; A. William Rutherford

doi:10.1016/0005-2728(87)90065-X

Formation and flash-dependent oscillation of the S₂-state multiline EPR signal in an oxygen-evolving Photosystem-II preparation lacking the three extrinsic proteins in the oxygen-evolving system

Stenbjörn Styring, Mitsue Miyao, A. William Rutherford

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

In PS-II-enriched membranes lacking the three extrinsic water-soluble proteins in the oxygen-evolving system (18, 24 and 33 kDa), but still evolving oxygen to some extent, the formation of the multiline EPR signal originating from the S₂-state is dependent on the concentration of Cl^-. In 200 mM Cl^- the multiline signal was observed after the first flash and oscillated with the flash number with a period of four. At 20 mM Cl^- no signal could be observed in this material. These results suggest that the extrinsic proteins are not necessary for multiline signal formation and that complete advancement through the S-states can occur in their absence when sufficient Cl^- is present.

Original language	English
Pages (from-to)	32-38
Number of pages	7
Journal	BBA - Bioenergetics
Volume	890
Issue number	1
DOIs	https://doi.org/10.1016/0005-2728(87)90065-X
Publication status	Published - 1987 Jan 16
Externally published	Yes

Keywords

(Spinach chloroplast)
ESR
Oxygen evolution
Photosystem II
S-state

ASJC Scopus subject areas

Biophysics
Biochemistry
Cell Biology

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10.1016/0005-2728(87)90065-X

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Formation and flash-dependent oscillation of the S₂-state multiline EPR signal in an oxygen-evolving Photosystem-II preparation lacking the three extrinsic proteins in the oxygen-evolving system. / Styring, Stenbjörn; Miyao, Mitsue; Rutherford, A. William.

In: BBA - Bioenergetics, Vol. 890, No. 1, 16.01.1987, p. 32-38.

Research output: Contribution to journal › Article › peer-review

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abstract = "In PS-II-enriched membranes lacking the three extrinsic water-soluble proteins in the oxygen-evolving system (18, 24 and 33 kDa), but still evolving oxygen to some extent, the formation of the multiline EPR signal originating from the S2-state is dependent on the concentration of Cl-. In 200 mM Cl- the multiline signal was observed after the first flash and oscillated with the flash number with a period of four. At 20 mM Cl- no signal could be observed in this material. These results suggest that the extrinsic proteins are not necessary for multiline signal formation and that complete advancement through the S-states can occur in their absence when sufficient Cl- is present.",

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