Formation and flash-dependent oscillation of the S2-state multiline EPR signal in an oxygen-evolving Photosystem-II preparation lacking the three extrinsic proteins in the oxygen-evolving system

Stenbjörn Styring, Mitsue Miyao, A. William Rutherford

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)

Abstract

In PS-II-enriched membranes lacking the three extrinsic water-soluble proteins in the oxygen-evolving system (18, 24 and 33 kDa), but still evolving oxygen to some extent, the formation of the multiline EPR signal originating from the S2-state is dependent on the concentration of Cl-. In 200 mM Cl- the multiline signal was observed after the first flash and oscillated with the flash number with a period of four. At 20 mM Cl- no signal could be observed in this material. These results suggest that the extrinsic proteins are not necessary for multiline signal formation and that complete advancement through the S-states can occur in their absence when sufficient Cl- is present.

Original languageEnglish
Pages (from-to)32-38
Number of pages7
JournalBBA - Bioenergetics
Volume890
Issue number1
DOIs
Publication statusPublished - 1987 Jan 16
Externally publishedYes

Keywords

  • (Spinach chloroplast)
  • ESR
  • Oxygen evolution
  • Photosystem II
  • S-state

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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