TY - JOUR
T1 - Formation and flash-dependent oscillation of the S2-state multiline EPR signal in an oxygen-evolving Photosystem-II preparation lacking the three extrinsic proteins in the oxygen-evolving system
AU - Styring, Stenbjörn
AU - Miyao, Mitsue
AU - Rutherford, A. William
N1 - Funding Information:
S.S. is grateful for a postdoctoral grant from the Knut and Alice Wallenbergs foundation. A.W.R. is supported by the CNRS. M.M. was supported by a Grant-in-Aid for Science Program for International Cooperative Research (60041101) from the Japanese Ministry of Education, Science and Culture.
PY - 1987/1/16
Y1 - 1987/1/16
N2 - In PS-II-enriched membranes lacking the three extrinsic water-soluble proteins in the oxygen-evolving system (18, 24 and 33 kDa), but still evolving oxygen to some extent, the formation of the multiline EPR signal originating from the S2-state is dependent on the concentration of Cl-. In 200 mM Cl- the multiline signal was observed after the first flash and oscillated with the flash number with a period of four. At 20 mM Cl- no signal could be observed in this material. These results suggest that the extrinsic proteins are not necessary for multiline signal formation and that complete advancement through the S-states can occur in their absence when sufficient Cl- is present.
AB - In PS-II-enriched membranes lacking the three extrinsic water-soluble proteins in the oxygen-evolving system (18, 24 and 33 kDa), but still evolving oxygen to some extent, the formation of the multiline EPR signal originating from the S2-state is dependent on the concentration of Cl-. In 200 mM Cl- the multiline signal was observed after the first flash and oscillated with the flash number with a period of four. At 20 mM Cl- no signal could be observed in this material. These results suggest that the extrinsic proteins are not necessary for multiline signal formation and that complete advancement through the S-states can occur in their absence when sufficient Cl- is present.
KW - (Spinach chloroplast)
KW - ESR
KW - Oxygen evolution
KW - Photosystem II
KW - S-state
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U2 - 10.1016/0005-2728(87)90065-X
DO - 10.1016/0005-2728(87)90065-X
M3 - Article
AN - SCOPUS:0342400336
VL - 890
SP - 32
EP - 38
JO - Biochimica et Biophysica Acta - Bioenergetics
JF - Biochimica et Biophysica Acta - Bioenergetics
SN - 0005-2728
IS - 1
ER -