Folding RNA–protein complex into designed nanostructures

Tomonori Shibata; Yuki Suzuki; Hiroshi Sugiyama; Masayuki Endo; Hirohide Saito

doi:10.1007/978-1-4939-2730-2_14

Folding RNA–protein complex into designed nanostructures

Tomonori Shibata, Yuki Suzuki, Hiroshi Sugiyama, Masayuki Endo, Hirohide Saito

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

RNA–protein (RNP) complexes are promising biomaterials for the fields of nanotechnology and synthetic biology. Protein-responsive RNA sequences (RNP motifs) can be integrated into various RNAs, such as messenger RNA, short-hairpin RNA, and synthetic RNA nano-objects for a variety of purposes. Direct observation of RNP interaction in solution at high resolution is important in the design and construction of RNP-mediated nanostructures. Here we describe a method to construct and visualize RNP nanostructures that precisely arrange a target protein on the RNA scaffold with nanometer scale. High-speed AFM (HS-AFM) images of RNP nanostructures show that the folding of RNP complexes of defined sizes can be directly visualized at single RNP resolution in solution.

Original language	English
Pages (from-to)	169-179
Number of pages	11
Journal	Methods in Molecular Biology
Volume	1316
DOIs	https://doi.org/10.1007/978-1-4939-2730-2_14
Publication status	Published - 2015 May 16
Externally published	Yes

Keywords

High-speed atomic force microscopy
Kink-turn RNA
L7Ae
RNA nanostructures
RNA nanotechnology
RNA–protein complex
RNP
Ribonucleoprotein

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1007/978-1-4939-2730-2_14

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title = "Folding RNA–protein complex into designed nanostructures",

abstract = "RNA–protein (RNP) complexes are promising biomaterials for the fields of nanotechnology and synthetic biology. Protein-responsive RNA sequences (RNP motifs) can be integrated into various RNAs, such as messenger RNA, short-hairpin RNA, and synthetic RNA nano-objects for a variety of purposes. Direct observation of RNP interaction in solution at high resolution is important in the design and construction of RNP-mediated nanostructures. Here we describe a method to construct and visualize RNP nanostructures that precisely arrange a target protein on the RNA scaffold with nanometer scale. High-speed AFM (HS-AFM) images of RNP nanostructures show that the folding of RNP complexes of defined sizes can be directly visualized at single RNP resolution in solution.",

keywords = "High-speed atomic force microscopy, Kink-turn RNA, L7Ae, RNA nanostructures, RNA nanotechnology, RNA–protein complex, RNP, Ribonucleoprotein",

author = "Tomonori Shibata and Yuki Suzuki and Hiroshi Sugiyama and Masayuki Endo and Hirohide Saito",

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AU - Shibata, Tomonori

AU - Suzuki, Yuki

AU - Sugiyama, Hiroshi

AU - Endo, Masayuki

AU - Saito, Hirohide

N1 - Publisher Copyright: © Springer Science+Business Media New York 2015.

PY - 2015/5/16

Y1 - 2015/5/16

N2 - RNA–protein (RNP) complexes are promising biomaterials for the fields of nanotechnology and synthetic biology. Protein-responsive RNA sequences (RNP motifs) can be integrated into various RNAs, such as messenger RNA, short-hairpin RNA, and synthetic RNA nano-objects for a variety of purposes. Direct observation of RNP interaction in solution at high resolution is important in the design and construction of RNP-mediated nanostructures. Here we describe a method to construct and visualize RNP nanostructures that precisely arrange a target protein on the RNA scaffold with nanometer scale. High-speed AFM (HS-AFM) images of RNP nanostructures show that the folding of RNP complexes of defined sizes can be directly visualized at single RNP resolution in solution.

AB - RNA–protein (RNP) complexes are promising biomaterials for the fields of nanotechnology and synthetic biology. Protein-responsive RNA sequences (RNP motifs) can be integrated into various RNAs, such as messenger RNA, short-hairpin RNA, and synthetic RNA nano-objects for a variety of purposes. Direct observation of RNP interaction in solution at high resolution is important in the design and construction of RNP-mediated nanostructures. Here we describe a method to construct and visualize RNP nanostructures that precisely arrange a target protein on the RNA scaffold with nanometer scale. High-speed AFM (HS-AFM) images of RNP nanostructures show that the folding of RNP complexes of defined sizes can be directly visualized at single RNP resolution in solution.

KW - High-speed atomic force microscopy

KW - Kink-turn RNA

KW - L7Ae

KW - RNA nanostructures

KW - RNA nanotechnology

KW - RNA–protein complex

KW - RNP

KW - Ribonucleoprotein

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Folding RNA–protein complex into designed nanostructures

Abstract

Keywords

ASJC Scopus subject areas

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