Folding RNA–protein complex into designed nanostructures

Tomonori Shibata, Yuki Suzuki, Hiroshi Sugiyama, Masayuki Endo, Hirohide Saito

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

RNA–protein (RNP) complexes are promising biomaterials for the fields of nanotechnology and synthetic biology. Protein-responsive RNA sequences (RNP motifs) can be integrated into various RNAs, such as messenger RNA, short-hairpin RNA, and synthetic RNA nano-objects for a variety of purposes. Direct observation of RNP interaction in solution at high resolution is important in the design and construction of RNP-mediated nanostructures. Here we describe a method to construct and visualize RNP nanostructures that precisely arrange a target protein on the RNA scaffold with nanometer scale. High-speed AFM (HS-AFM) images of RNP nanostructures show that the folding of RNP complexes of defined sizes can be directly visualized at single RNP resolution in solution.

Original languageEnglish
Pages (from-to)169-179
Number of pages11
JournalMethods in Molecular Biology
Volume1316
DOIs
Publication statusPublished - 2015 May 16
Externally publishedYes

Keywords

  • High-speed atomic force microscopy
  • Kink-turn RNA
  • L7Ae
  • RNA nanostructures
  • RNA nanotechnology
  • RNA–protein complex
  • RNP
  • Ribonucleoprotein

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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