Abstract
RNA–protein (RNP) complexes are promising biomaterials for the fields of nanotechnology and synthetic biology. Protein-responsive RNA sequences (RNP motifs) can be integrated into various RNAs, such as messenger RNA, short-hairpin RNA, and synthetic RNA nano-objects for a variety of purposes. Direct observation of RNP interaction in solution at high resolution is important in the design and construction of RNP-mediated nanostructures. Here we describe a method to construct and visualize RNP nanostructures that precisely arrange a target protein on the RNA scaffold with nanometer scale. High-speed AFM (HS-AFM) images of RNP nanostructures show that the folding of RNP complexes of defined sizes can be directly visualized at single RNP resolution in solution.
Original language | English |
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Pages (from-to) | 169-179 |
Number of pages | 11 |
Journal | Methods in Molecular Biology |
Volume | 1316 |
DOIs | |
Publication status | Published - 2015 May 16 |
Externally published | Yes |
Keywords
- High-speed atomic force microscopy
- Kink-turn RNA
- L7Ae
- RNA nanostructures
- RNA nanotechnology
- RNA–protein complex
- RNP
- Ribonucleoprotein
ASJC Scopus subject areas
- Molecular Biology
- Genetics