Fluorometric determination of sugars using fluorescein-labeled concanavalin A-glycogen conjugates

Katsuhiko Sato, Jun Ichi Anzai

Research output: Contribution to journalArticlepeer-review

36 Citations (Scopus)

Abstract

The fluorescence of fluoresceinisothiocyanate-labeled concanavalin A (FITC-Con A) was quenched by forming an FITC-Con A-glycogen conjugate and dequenched upon addition of sugars to the conjugate solution due to disaggregation of the conjugate. However, fluorescence quenching was barely observed upon formation of FITC-Con A-dextran conjugate. The sugar-induced fluorescence response of the FITC-Con A-glycogen conjugate depended significantly on the type of sugar: methylated α-D-glucose and α-D-mannose both induced high and rapid responses, while the responses to D-mannose and D-glucose were moderate. In contrast, no response was observed in the presence of D-galactose due to a lack of affinity to Con A. Thus, it is apparent that D-glucose and other sugars can be detected via the fluorescence of the FITC-Con A-glycogen conjugate.

Original languageEnglish
Pages (from-to)1297-1301
Number of pages5
JournalAnalytical and Bioanalytical Chemistry
Volume384
Issue number6
DOIs
Publication statusPublished - 2006 Mar

Keywords

  • Con A-glycogen conjugate
  • Concanavalin A
  • D-glucose detection
  • Fluorescence
  • Glycogen

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

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