Fluorometric determination of serum and urinary aluminium with 8- quinolinol by kinetic-differentiation-mode micellar chromatography

Makoto Sato, Hajime Yoshimura, Tetsuo Shimmura, Hideki Obi, Hatakeyama Shin-Ichi Hatakeyama, Emiko Kaneko, Hitoshi Hoshino, Takao Yotsuyanagi

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

A new fluorometric method has been developed for the determination of aluminium with 8-quinolinol by kinetic-differentiation-mode micellar chromatography. The proposed method enabled the determination of aluminium down to 1 μg/l inhuman serum and urine without preliminary deproteinization. The most remarkable point of this method is that only aluminium ion selectively responds among metal ions. The complex formation of aluminium in serum with 8-quinolinol was completed within a few minutes at room temperature. The serum matrix and aluminium chelate were separated on a reversed-phase column with an eluent containing acetonitrile, sodium dodecylsulfate, and Triton X-100, and aluminium chelate was detected at Ex 370 nm, Em 504 nm. The values obtained by this method were in good agreement with those of Zeeman graphite-furnace atomic absorption spectrometry. The proposed method will provide a simple and rapid technique for the determination aluminium in medical fields.

Original languageEnglish
Pages (from-to)361-367
Number of pages7
JournalJournal of Chromatography A
Volume789
Issue number1-2
DOIs
Publication statusPublished - 1997 Nov 21

Keywords

  • Aluminium
  • Kinetic differentiation liquid chromatography
  • Metal chelates
  • Micellar liquid chromatography
  • Quinolinol
  • Serum
  • Urine

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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    Sato, M., Yoshimura, H., Shimmura, T., Obi, H., Shin-Ichi Hatakeyama, H., Kaneko, E., Hoshino, H., & Yotsuyanagi, T. (1997). Fluorometric determination of serum and urinary aluminium with 8- quinolinol by kinetic-differentiation-mode micellar chromatography. Journal of Chromatography A, 789(1-2), 361-367. https://doi.org/10.1016/S0021-9673(97)00510-4