Keeping quenchers on a short leash: Shortening of the linker chain length combined with modification of the quencher moiety was found to improve fluorogenic probes useful for protein labeling by the mutant β-lactamase-tag (BL-tag) technology. The most effective probe, CC3 DNB, displays fluorescence quenching by photoinduced electron transfer (PET) and results in fluorescent protein labeling with fast kinetics.
- electron transfer
- fluorescence spectroscopy
- fluorescent probesprotein labeling
ASJC Scopus subject areas
- Organic Chemistry