Fluorogenic protein labeling through photoinduced electron transfer-based BL-tag technology

Kalyan K. Sadhu, Shin Mizukami, Carolyn R. Lanam, Kazuya Kikuchi

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Keeping quenchers on a short leash: Shortening of the linker chain length combined with modification of the quencher moiety was found to improve fluorogenic probes useful for protein labeling by the mutant β-lactamase-tag (BL-tag) technology. The most effective probe, CC3 DNB, displays fluorescence quenching by photoinduced electron transfer (PET) and results in fluorescent protein labeling with fast kinetics.

Original languageEnglish
Pages (from-to)272-276
Number of pages5
JournalChemistry - An Asian Journal
Volume7
Issue number2
DOIs
Publication statusPublished - 2012 Feb 6
Externally publishedYes

Keywords

  • biotechnology
  • electron transfer
  • fluorescence spectroscopy
  • fluorescent probesprotein labeling

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry

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